| Literature DB >> 27975283 |
Mehdi Mirzaei1, Dana Pascovici2, Jemma X Wu2, Joel Chick3, Yunqi Wu1, Brett Cooke2, Paul Haynes1, Mark P Molloy4,5.
Abstract
In this chapter we describe the workflow we use for labeled quantitative proteomics analysis using tandem mass tags (TMT) starting with the sample preparation and ending with the multivariate analysis of the resulting data. We detail the step-by-step process from sample processing, labeling, fractionation, and data processing using Proteome Discoverer through to data analysis and interpretation in the context of a multi-run experiment. The final analysis and data interpretation rely on an R package we call TMTPrepPro, which are deployed on a local GenePattern server, and used for generating various outputs which are also outlined herein.Keywords: Quantitative shotgun proteomics; Software workflow; TMT
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Year: 2017 PMID： 27975283 DOI： 10.1007/978-1-4939-6740-7_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745