| Literature DB >> 27975256 |
Karen Hoppens Gylys1, Tina Bilousova2.
Abstract
Synaptosomes, resealed nerve terminals that form when tissue is homogenized in isotonic medium, are a model system that has been a key source of knowledge about neurotransmission. Synaptosomes contain mitochondria, cytoskeletal proteins, and release neurotransmitters; many have postsynaptic elements. Cryopreservation at the time of autopsy makes it possible to prepare synaptosomes from human samples. Flow cytometry is a powerful analytic technique that precisely measures fluorescence on a cell-by-cell basis, and also indicates particle size and complexity with a routine parameter that measures light scattering. We describe here a procedure for flow cytometry analysis of tau in synaptosomes, a procedure that enables (1) "purification" of synaptosomes from the P-2 fraction (crude synaptosomes) by gating on particle size, and (2) quantitative measure of tau immunofluorescence in individual terminals. Application of flow cytometry to study of synaptosomes has yielded important information, not possible with routine biochemistry, about synaptic pathology in Alzheimer's disease.Entities:
Keywords: Cytoskeleton; FACs; Flow cytometry; HT7 antibody; Neurodegeneration; Tau fragment
Mesh：
Substances：
Year: 2017 PMID： 27975256 PMCID： PMC5755961 DOI： 10.1007/978-1-4939-6598-4_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745