| Literature DB >> 27975215 |
Kenneth Verheggen1,2, Lennart Martens1,2, Frode S Berven3,4,5, Harald Barsnes6, Marc Vaudel3.
Abstract
The first step in identifying proteins from mass spectrometry based shotgun proteomics data is to infer peptides from tandem mass spectra, a task generally achieved using database search engines. In this chapter, the basic principles of database search engines are introduced with a focus on open source software, and the use of database search engines is demonstrated using the freely available SearchGUI interface. This chapter also discusses how to tackle general issues related to sequence database searching and shows how to minimize their impact.Keywords: Peptide identification; Search engines; Sequence database searching; Shotgun proteomics
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Year: 2016 PMID： 27975215 DOI： 10.1007/978-3-319-41448-5_6
Source DB: PubMed Journal: Adv Exp Med Biol ISSN： 0065-2598 Impact factor: 2.622