Literature DB >> 27974227

Evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of Bordetella pertussis infection.

Kazunari Kamachi1, Takumi Moriuchi2, Yukihiro Hiramatsu3, Nao Otsuka3, Keigo Shibayama3.   

Abstract

We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bordetella pertussis; Loop-meditated isothermal amplification (LAMP); Pertussis toxin promoter; ptxP1; ptxP3

Mesh：

Substances：

Year:  2016        PMID： 27974227     DOI： 10.1016/j.mimet.2016.12.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  2 in total

Review 1.  Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.

Authors:  Y-P Wong; S Othman; Y-L Lau; S Radu; H-Y Chee
Journal:  J Appl Microbiol       Date:  2018-02-12       Impact factor: 3.772

2.  Severe pertussis in a young infant due to household transmission: the needs of pertussis vaccination boosters in Japan.

Authors:  Daiji Takajo; Shigeaki Nonoyama
Journal:  Clin Case Rep       Date:  2018-03-08
  2 in total

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