| Literature DB >> 2796783 |
Abstract
It is established that the use of DNA-dependent RNA-polymerases of mollicutes in the molecular-biological and gene-engineering works aimed to obtain a sufficient amount of the transcription products may promote a 1.5-2-fold increase of the yield by introducing such substances as glycerol, dimethylsulphoxide and spermidine (optimal concentrations: 10-12 vol. % and 12 mM, respectively) into the composition of the reacting mixture.Entities:
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Year: 1989 PMID: 2796783
Source DB: PubMed Journal: Mikrobiol Zh ISSN: 0201-8462