Literature DB >> 2796783

[Increase in the in vitro activity of mollicute transcriptase using substances of an organic nature].

I G Skripal', V V Babichev.   

Abstract

It is established that the use of DNA-dependent RNA-polymerases of mollicutes in the molecular-biological and gene-engineering works aimed to obtain a sufficient amount of the transcription products may promote a 1.5-2-fold increase of the yield by introducing such substances as glycerol, dimethylsulphoxide and spermidine (optimal concentrations: 10-12 vol. % and 12 mM, respectively) into the composition of the reacting mixture.

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Year:  1989        PMID: 2796783

Source DB:  PubMed          Journal:  Mikrobiol Zh        ISSN: 0201-8462


  2 in total

1.  In vivo interpretation of in vitro effect studies with a detailed analysis of the method of in vitro transcription in isolated cell nuclei.

Authors:  R Strand; R Fjelland; T Flatmark
Journal:  Acta Biotheor       Date:  1996-03       Impact factor: 1.774

2.  The choice of resuspension medium for isolated rat liver nuclei: effects on nuclear morphology and in vitro transcription.

Authors:  R Strand; R Bøe; T Flatmark
Journal:  Mol Cell Biochem       Date:  1994-10-26       Impact factor: 3.396

  2 in total

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