| Literature DB >> 27966701 |
M Li1, H L Lightfoot1, F Halloy1, A L Malinowska1, C Berk1, A Behera1, D Schümperli2, J Hall1.
Abstract
Stereochemically-pure 2'-O-(2-methoxyethyl)-phosphorothioate (PS-MOE) oligonucleotides were synthesized from new chiral oxazaphospholidine-containing nucleosides. Thermal stability studies showed that the incorporation of Rp-PS linkages increased RNA-binding affinity. In cells, a full Rp-PS-MOE splice-switching oligonucleotide targeting part of the ferrochelatase gene was more potent than its Sp-PS counterpart, but of similar potency to the stereorandom PS-parent sequence.Entities:
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Year: 2017 PMID： 27966701 DOI： 10.1039/c6cc08473g
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222