| Literature DB >> 27965121 |
I F Pinto1, C R F Caneira2, R R G Soares1, N Madaboosi2, M R Aires-Barros3, J P Conde4, A M Azevedo3, V Chu5.
Abstract
This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.Entities:
Keywords: Antibodies; Biosensing; Microbeads; Microfluidics; Mycotoxins; Nucleic acids
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Year: 2016 PMID： 27965121 DOI： 10.1016/j.ymeth.2016.12.005
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608