| Literature DB >> 27965064 |
Elena Mellado-Ortega1, Iñigo Zabalgogeazcoa2, Beatriz R Vázquez de Aldana3, Juan B Arellano4.
Abstract
Oxygen radical absorbance capacity (ORAC) assay in 96-well multi-detection plate readers is a rapid method to determine total antioxidant capacity (TAC) in biological samples. A disadvantage of this method is that the antioxidant inhibition reaction does not start in all of the 96 wells at the same time due to technical limitations when dispensing the free radical-generating azo initiator 2,2'-azobis (2-methyl-propanimidamide) dihydrochloride (AAPH). The time delay between wells yields a systematic error that causes statistically significant differences in TAC determination of antioxidant solutions depending on their plate position. We propose two alternative solutions to avoid this AAPH-dependent error in ORAC assays.Entities:
Keywords: 96-Well plate reader; AAPH; ORAC; Peroxyl radical; Total antioxidant capacity; Trolox
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Year: 2016 PMID： 27965064 DOI： 10.1016/j.ab.2016.12.009
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365