Literature DB >> 27943204

Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.

Sybren L N Maas1,2, Marike L D Broekman1,2, Jeroen de Vrij3,4,5.   

Abstract

Accurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise as a method for single particle-based quantification and size profiling of EVs. Here, we describe the technical background of tRPS and its applications for EV characterization. Besides the standard protocol, we describe an alternative protocol, in which samples are spiked with polystyrene beads of known size and concentration. This alternative protocol can be used to overcome some of the challenges of direct EV characterization in biological fluids.

Entities:  

Keywords:  Characterization; Exosomes; Extracellular vesicles; Microvesicles; Quantification; Resistive pulse sensing; Size distribution; qNano

Mesh：

Year:  2017        PMID： 27943204     DOI： 10.1007/978-1-4939-6728-5_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  24 in total

Review 1.  Biosensors for Detection of Human Placental Pathologies: A Review of Emerging Technologies and Current Trends.

Authors:  Jia Liu; Babak Mosavati; Andrew V Oleinikov; E Du
Journal:  Transl Res       Date:  2019-05-20       Impact factor: 7.012

2.  Elevated exosomal secretion of miR-124-3p from spinal neurons positively associates with disease severity in ALS.

Authors:  Julia Yelick; Yuqin Men; Shijie Jin; Sabrina Seo; Francisco Espejo-Porras; Yongjie Yang
Journal:  Exp Neurol       Date:  2020-07-24       Impact factor: 5.330

Review 3.  Ceramide and Exosomes: A Novel Target in Cancer Biology and Therapy.

Authors:  Ahmed Elsherbini; Erhard Bieberich
Journal:  Adv Cancer Res       Date:  2018-06-09       Impact factor: 6.242

4.  Submicron Protein Particle Characterization using Resistive Pulse Sensing and Conventional Light Scattering Based Approaches.

Authors:  Gregory V Barnett; Julia M Perhacs; Tapan K Das; Sambit R Kar
Journal:  Pharm Res       Date:  2018-02-08       Impact factor: 4.200

Review 5.  Circulating extracellular vesicles and rheumatoid arthritis: a proteomic analysis.

Authors:  Mohammed Alghamdi; Sultan Abdulmughni Alamry; Sami M Bahlas; Vladimir N Uversky; Elrashdy M Redwan
Journal:  Cell Mol Life Sci       Date:  2021-12-31       Impact factor: 9.261

Review 6.  Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation.

Authors:  Nna-Emeka Onukwugha; Yoon-Tae Kang; Sunitha Nagrath
Journal:  Lab Chip       Date:  2022-09-13       Impact factor: 7.517

Review 7.  New Technologies for Analysis of Extracellular Vesicles.

Authors:  Huilin Shao; Hyungsoon Im; Cesar M Castro; Xandra Breakefield; Ralph Weissleder; Hakho Lee
Journal:  Chem Rev       Date:  2018-01-31       Impact factor: 60.622

8.  Insights into the Proteome of Gastrointestinal Stromal Tumors-Derived Exosomes Reveals New Potential Diagnostic Biomarkers.

Authors:  Safinur Atay; Daniel W Wilkey; Mohammed Milhem; Michael Merchant; Andrew K Godwin
Journal:  Mol Cell Proteomics       Date:  2017-12-14       Impact factor: 5.911

Review 9.  Extracellular Vesicles as an Emerging Treatment Option for Intervertebral Disc Degeneration: Therapeutic Potential, Translational Pathways, and Regulatory Considerations.

Authors:  Tyler J DiStefano; Keti Vaso; George Danias; Henry N Chionuma; Jennifer R Weiser; James C Iatridis
Journal:  Adv Healthc Mater       Date:  2021-07-23       Impact factor: 9.933

Review 10.  The Mystery of Red Blood Cells Extracellular Vesicles in Sleep Apnea with Metabolic Dysfunction.

Authors:  Abdelnaby Khalyfa; David Sanz-Rubio
Journal:  Int J Mol Sci       Date:  2021-04-21       Impact factor: 5.923
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