Literature DB >> 27943203

Methods to Analyze EVs.

Bernd Giebel1, Clemens Helmbrecht2.   

Abstract

Research in the field of extracellular vesicles (EVs) is challenged by the small size of the nano-sized particles. Apart from the use of transmission and scanning electron microscopy, established technical platforms to visualize, quantify, and characterize nano-sized EVs were lacking. Recently, methodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.

Keywords:  Dynamic light scattering; Electron microscopy; Extracellular vesicles; Flow cytometry; Nanoparticle tracking analysis; Resistive pulse sensing

Mesh：

Substances：

Year:  2017        PMID： 27943203     DOI： 10.1007/978-1-4939-6728-5_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  Extracellular Vesicles in Pathogenesis and Treatment of Metabolic Associated Fatty Liver Disease.

Authors:  Ji Sun; Dianbao Zhang; Yiling Li
Journal:  Front Physiol       Date:  2022-06-13       Impact factor: 4.755

Review 2.  Recent advances in optical label-free characterization of extracellular vesicles.

Authors:  Meruyert Imanbekova; Sorina Suarasan; Yao Lu; Sarah Jurchuk; Sebastian Wachsmann-Hogiu
Journal:  Nanophotonics       Date:  2022-04-13       Impact factor: 7.923

Review 3.  Challenges in the Isolation and Proteomic Analysis of Cancer Exosomes-Implications for Translational Research.

Authors:  Jadwiga Jablonska; Monika Pietrowska; Sonja Ludwig; Stephan Lang; Basant Kumar Thakur
Journal:  Proteomes       Date:  2019-05-15

4.  Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications.

Authors:  Kenneth W Witwer; Bas W M Van Balkom; Stefania Bruno; Andre Choo; Massimo Dominici; Mario Gimona; Andrew F Hill; Dominique De Kleijn; Mickey Koh; Ruenn Chai Lai; S Alex Mitsialis; Luis A Ortiz; Eva Rohde; Takashi Asada; Wei Seong Toh; Daniel J Weiss; Lei Zheng; Bernd Giebel; Sai Kiang Lim
Journal:  J Extracell Vesicles       Date:  2019-04-29

Review 5.  Functional assays to assess the therapeutic potential of extracellular vesicles.

Authors:  Vivian V T Nguyen; Kenneth W Witwer; Marianne C Verhaar; Dirk Strunk; Bas W M van Balkom
Journal:  J Extracell Vesicles       Date:  2020-11-29

6.  Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms.

Authors:  Tanina Arab; Emily R Mallick; Yiyao Huang; Liang Dong; Zhaohao Liao; Zezhou Zhao; Olesia Gololobova; Barbara Smith; Norman J Haughey; Kenneth J Pienta; Barbara S Slusher; Patrick M Tarwater; Juan Pablo Tosar; Angela M Zivkovic; Wyatt N Vreeland; Michael E Paulaitis; Kenneth W Witwer
Journal:  J Extracell Vesicles       Date:  2021-04-06

7.  Label-free characterization of an extracellular vesicle-based therapeutic.

Authors:  Eleni Priglinger; Juergen Strasser; Boris Buchroithner; Florian Weber; Susanne Wolbank; Daniela Auer; Eva Grasmann; Claudia Arzt; Dmitry Sivun; Johannes Grillari; Jaroslaw Jacak; Johannes Preiner; Mario Gimona
Journal:  J Extracell Vesicles       Date:  2021-10

8.  Detailed Characterization of Small Extracellular Vesicles from Different Cell Types Based on Tetraspanin Composition by ExoView R100 Platform.

Authors:  Kai Breitwieser; Leon F Koch; Tobias Tertel; Eva Proestler; Luisa D Burgers; Christoph Lipps; James Adjaye; Robert Fürst; Bernd Giebel; Meike J Saul
Journal:  Int J Mol Sci       Date:  2022-08-01       Impact factor: 6.208

9.  Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients.

Authors:  Tobias Tertel; Sergej Tomić; Jelena Đokić; Dušan Radojević; Dejan Stevanović; Nataša Ilić; Bernd Giebel; Maja Kosanović
Journal:  J Extracell Vesicles       Date:  2022-08

Review 10.  Liquid biomarkers in melanoma: detection and discovery.

Authors:  Su Yin Lim; Jenny H Lee; Russell J Diefenbach; Richard F Kefford; Helen Rizos
Journal:  Mol Cancer       Date:  2018-01-17       Impact factor: 27.401
  10 in total

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