Joshua Weiner1, Julien Zuber, Brittany Shonts, Suxiao Yang, Jianing Fu, Mercedes Martinez, Donna L Farber, Tomoaki Kato, Megan Sykes. 1. 1 Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center, Columbia University, New York, NY. 2 Department of Surgery, Columbia University, New York, NY. 3 Department of Pediatrics, Division of Gastroenterology, Columbia University, New York, NY. 4 Department of Microbiology and Immunology, Columbia University Medical Center, Columbia University, New York, NY 10032, USA.
Abstract
BACKGROUND: Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described. METHODS: Intestinal samples were taken from 4 isolated intestine and 3 multivisceral transplant recipients at the time of any operative resection, such as stoma closure or revision. ILCs were isolated and analyzed by flow cytometry. The target population was defined as being negative for lineage markers and double-positive for CD45/CD127. Cells were further stained to define ILC subsets and a donor-specific or recipient-specific HLA marker to analyze chimerism. RESULTS: Donor-derived ILCs were found to persist greater than 8 years after transplantation. Additionally, the percentage of cells thought to be lymphoid tissue inducer cells among donor ILCs was far higher than that among recipient ILCs. CONCLUSIONS: Our findings demonstrate that donor-derived ILCs persist long-term after transplantation and support the notion that human lymphoid tissue inducer cells may form in the fetus and persist throughout life, as hypothesized in rodents. Correlation between chimerism and rejection, graft failure, and patient survival requires further study.
BACKGROUND: Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described. METHODS: Intestinal samples were taken from 4 isolated intestine and 3 multivisceral transplant recipients at the time of any operative resection, such as stoma closure or revision. ILCs were isolated and analyzed by flow cytometry. The target population was defined as being negative for lineage markers and double-positive for CD45/CD127. Cells were further stained to define ILC subsets and a donor-specific or recipient-specific HLA marker to analyze chimerism. RESULTS: Donor-derived ILCs were found to persist greater than 8 years after transplantation. Additionally, the percentage of cells thought to be lymphoid tissue inducer cells among donor ILCs was far higher than that among recipient ILCs. CONCLUSIONS: Our findings demonstrate that donor-derived ILCs persist long-term after transplantation and support the notion that human lymphoid tissue inducer cells may form in the fetus and persist throughout life, as hypothesized in rodents. Correlation between chimerism and rejection, graft failure, and patient survival requires further study.
Authors: T E Starzl; A J Demetris; M Trucco; N Murase; C Ricordi; S Ildstad; H Ramos; S Todo; A Tzakis; J J Fung Journal: Hepatology Date: 1993-06 Impact factor: 17.425
Authors: Tom Cupedo; Natasha K Crellin; Natalie Papazian; Elwin J Rombouts; Kees Weijer; Jane L Grogan; Willem E Fibbe; Jan J Cornelissen; Hergen Spits Journal: Nat Immunol Date: 2008-11-23 Impact factor: 25.606
Authors: Rimma Goldberg; Natalie Prescott; Graham M Lord; Thomas T MacDonald; Nick Powell Journal: Nat Rev Gastroenterol Hepatol Date: 2015-05 Impact factor: 46.802
Authors: Jiman Kang; Katrina Loh; Leonid Belyayev; Priscilla Cha; Mohammed Sadat; Khalid Khan; Yuriy Gusev; Krithika Bhuvaneshwar; Habtom Ressom; Sangeetha Moturi; Jason Kaiser; Jason Hawksworth; Simon C Robson; Cal S Matsumoto; Michael Zasloff; Thomas M Fishbein; Alexander Kroemer Journal: Am J Transplant Date: 2020-07-21 Impact factor: 8.086
Authors: Nadja Stobutzki; Stephan Schlickeiser; Mathias Streitz; Katarina Stanko; Kim-Long Truong; Levent Akyuez; Katrin Vogt; Christine Appelt; Andreas Pascher; Olga Blau; Undine A Gerlach; Birgit Sawitzki Journal: Front Immunol Date: 2019-05-07 Impact factor: 7.561
Authors: Patrice Zeis; Mi Lian; Xiying Fan; Josip S Herman; Daniela C Hernandez; Rebecca Gentek; Shlomo Elias; Cornelia Symowski; Konrad Knöpper; Nina Peltokangas; Christin Friedrich; Remi Doucet-Ladeveze; Agnieszka M Kabat; Richard M Locksley; David Voehringer; Marc Bajenoff; Alexander Y Rudensky; Chiara Romagnani; Dominic Grün; Georg Gasteiger Journal: Immunity Date: 2020-09-30 Impact factor: 31.745