Irene Shuping Zhao1, May Lei Mei2, Michael F Burrow3, Edward Chin-Man Lo4, Chun-Hung Chu5. 1. Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address: zhao110@hku.hk. 2. Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address: mei1123@hku.hk. 3. Melbourne Dental School, University of Melbourne, Melbourne, Australia. Electronic address: mfburrow@unimelb.edu.au. 4. Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address: hrdplcm@hku.hk. 5. Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address: chchu@hku.hk.
Abstract
OBJECTIVE: To study the effect of silver diamine fluoride (SDF) treatment and incorporating casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) into a glass-ionomer cement (GIC) to prevent secondary caries. METHOD: A cervical cavity was prepared on 32 premolars for the following restoration groups: group 1, conventional GIC restoration; group 2, SDF (38%) treatment and conventional GIC restoration; group 3, CPP-ACP (3%) modified GIC; and group 4, SDF treatment and CPP-ACP modified GIC. The restored teeth were thermal-cycled before undergoing a multi-species cariogenic biofilm challenge. The restored teeth were examined by micro-computed tomography (micro-CT), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX) and Fourier transform infrared (FTIR) spectroscopy. Data were analyzed by two-way ANOVA. RESULTS: Micro-CT determined outer lesion depths for groups 1-4 were: 123±6μm, 87±7μm, 79±3μm and 68±5μm respectively. An interaction effect on the outer lesion depth was found between the restorative materials and SDF treatment (p<0.001). Both SDF treatment and modification with CPP-ACP had a significant effect on outer lesion depth (p<0.001). SEM/EDX showed an increase of calcium and phosphorus at the root dentine adjacent to the restoration in groups 3 and 4 (CPP-ACP modified GIC). FTIR revealed that SDF treatment and CPP-ACP modified GIC had a significant effect on amide I-to-hydrogen phosphate ratio on the material-root interface (p=0.001). CONCLUSION: SDF treatment and incorporation of CPP-ACP into GIC restorative material can prevent secondary root caries development. CLINICAL SIGNIFICANCE: The results provide useful information to dentists in formulating clinical management protocols and material when treating root caries.
OBJECTIVE: To study the effect of silver diamine fluoride (SDF) treatment and incorporating casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) into a glass-ionomer cement (GIC) to prevent secondary caries. METHOD: A cervical cavity was prepared on 32 premolars for the following restoration groups: group 1, conventional GIC restoration; group 2, SDF (38%) treatment and conventional GIC restoration; group 3, CPP-ACP (3%) modified GIC; and group 4, SDF treatment and CPP-ACP modified GIC. The restored teeth were thermal-cycled before undergoing a multi-species cariogenic biofilm challenge. The restored teeth were examined by micro-computed tomography (micro-CT), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX) and Fourier transform infrared (FTIR) spectroscopy. Data were analyzed by two-way ANOVA. RESULTS: Micro-CT determined outer lesion depths for groups 1-4 were: 123±6μm, 87±7μm, 79±3μm and 68±5μm respectively. An interaction effect on the outer lesion depth was found between the restorative materials and SDF treatment (p<0.001). Both SDF treatment and modification with CPP-ACP had a significant effect on outer lesion depth (p<0.001). SEM/EDX showed an increase of calcium and phosphorus at the root dentine adjacent to the restoration in groups 3 and 4 (CPP-ACP modified GIC). FTIR revealed that SDF treatment and CPP-ACP modified GIC had a significant effect on amide I-to-hydrogen phosphate ratio on the material-root interface (p=0.001). CONCLUSION:SDF treatment and incorporation of CPP-ACP into GIC restorative material can prevent secondary root caries development. CLINICAL SIGNIFICANCE: The results provide useful information to dentists in formulating clinical management protocols and material when treating root caries.
Authors: John Yun Niu; Iris Xiaoxue Yin; William Ka Kei Wu; Quan-Li Li; May Lei Mei; Chun Hung Chu Journal: Clin Oral Investig Date: 2021-10-12 Impact factor: 3.573
Authors: Irene Shuping Zhao; Iris Xiaoxue Yin; May Lei Mei; Edward Chin Man Lo; Jinyao Tang; Quanli Li; Lok Yan So; Chun Hung Chu Journal: Int J Nanomedicine Date: 2020-04-23
Authors: Irene Shuping Zhao; Samantha Chu; Ollie Yiru Yu; May Lei Mei; Chun Hung Chu; Edward Chin Man Lo Journal: Int Dent J Date: 2019-03-20 Impact factor: 2.607
Authors: Irene Shuping Zhao; May Lei Mei; Zhuo Long Zhou; Michael Francis Burrow; Edward Chin-Man Lo; Chun-Hung Chu Journal: Int J Mol Sci Date: 2017-08-07 Impact factor: 5.923