Draft Genome Sequence of Probiotic Lactobacillus acidophilus Strain L-55 Isolated from a Healthy Human Gut.

Probiotic Lactobacillus acidophilus L-55 was isolated from a healthy human gut. Here, we report the draft genome sequence of this organism.

GENOME ANNOUNCEMENT

Lactobacillus acidophilus, which is a Gram-positive bacterium, is often used in dairy products as a probiotic lactic acid bacterium that benefits the health of the host (1, 2). L. acidophilus strain L-55, which was isolated from healthy infant feces (3, 4), has tolerance to gastrointestinal conditions and a high ability to adhere to Caco-2 cells (3, 4), and it has been used as a starter culture for commercial dairy products (3). It has also been reported that L. acidophilus L-55 has the effect of alleviating the symptoms of allergic rhinitis (3, 5) and atopic dermatitis (4).

The L. acidophilus L-55 genome was paired-end sequenced using Illumina’s MiSeq platform. Genomic libraries containing 600- to 1,000-bp inserts were constructed and sequenced, yielding 1,981,953 reads that provided 296-fold coverage of the genome. The sequence reads were assembled using the CLC Genomics Workbench version 9.0.1, and the assembled genome consists of 25 contigs with a total length of 2,009,507 bp. The genome has a G+C content of 34.6%. These features were very similar to those of 15 other sequenced L. acidophilus strains, except strain CFH, consistent with a previous report that L. acidophilus lacks genomic diversity compared to other L. acidophilus group species (6). The draft genome of L. acidophilus L-55 contained 1,884 predicted protein-coding genes, 1,760 (93%) of which were shared with the complete genome sequence of L. acidophilus NCFM (7). The genome information of this species will be useful for further studies of its physiology, taxonomy, and ecology.

Accession number(s).

The draft genome sequence for L. acidophilus L-55 has been deposited in the DDBJ/GenBank/EMBL database under accession numbers BDHM01000001 to BDHM01000025.