Lucine Vuitton1, Carine Jaillet2, Elise Jacquin3, Franck Monnien4, Marine Heberle4, Maria I Mihai4, Catherine Lassabe5, Johnny Raffoul5, Marc Puyraveau6, Zaher Lakkis7, Najib Lamfichekh8, Alain Picard8, Jean-Luc Prétet9, Christiane Mougin9, Séverine Valmary-Degano9. 1. Department of Gastroenterology, University Hospital of Besançon, Besançon, France; Bourgogne Franche-Comté University, EA3181, Besançon, France. Electronic address: lvuitton@chu-besancon.fr. 2. Bourgogne Franche-Comté University, EA3181, Besançon, France. 3. Bourgogne Franche-Comté University, EA3181, Besançon, France; Signalling Department, Babraham Institute, Cambridge, United Kingdom. 4. Department of Pathology, University Hospital of Besançon, Besançon, France. 5. Department of Pathology, Belfort-Montbéliard Hospital, France. 6. Centre de Méthodologie Clinique, University Hospital of Besançon, Besançon, France. 7. Department of Digestive and Oncologic Surgery - Liver Transplantation Unit, University Hospital of Besançon, Besançon, France. 8. Department of Sigestive Surgery, Belfort-Montbéliard Hospital, France. 9. Bourgogne Franche-Comté University, EA3181, Besançon, France; Department of Pathology, University Hospital of Besançon, Besançon, France.
Abstract
BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers. As in other cancer locations, the involvement of human papillomaviruses (HPV) has been suggested but remains highly debated with wide differences among reported prevalence of HPV infection in CRCs. AIM: To determine the actual prevalence of high risk HPV16 and 18 in a large case-control study. METHODS: CRC specimens were used for analysis of both tumor and distant healthy tissue. As a non-malignant control group, samples from sigmoid diverticulosis resections were studied. Detection of HPV16 and HPV18 DNA was performed using a real time polymerase chain reaction (qPCR). Ten percent of tumor samples were also randomly subjected to a complete HPV genotyping using the INNO-LiPA technique. RESULTS: 467 samples were analyzed: 217 tumor samples from 210 CRCs, 210 distant healthy tissue samples, and 40 sigmoid samples. HPV18 DNA was never amplified and HPV16 was amplified only three times in tumor tissues with viral loads under or at the limit of quantification. New extraction from the same tumor blocks for these samples revealed no HPV with qPCR and INNO-Lipa assays. CONCLUSION: With adequate procedures and reliable techniques, no HPV was detected in the largest case-control study so far, bringing more evidence on the absence of involvement of HPV in CRCs.
BACKGROUND:Colorectal cancer (CRC) is one of the most common cancers. As in other cancer locations, the involvement of human papillomaviruses (HPV) has been suggested but remains highly debated with wide differences among reported prevalence of HPV infection in CRCs. AIM: To determine the actual prevalence of high risk HPV16 and 18 in a large case-control study. METHODS:CRC specimens were used for analysis of both tumor and distant healthy tissue. As a non-malignant control group, samples from sigmoid diverticulosis resections were studied. Detection of HPV16 and HPV18 DNA was performed using a real time polymerase chain reaction (qPCR). Ten percent of tumor samples were also randomly subjected to a complete HPV genotyping using the INNO-LiPA technique. RESULTS: 467 samples were analyzed: 217 tumor samples from 210 CRCs, 210 distant healthy tissue samples, and 40 sigmoid samples. HPV18 DNA was never amplified and HPV16 was amplified only three times in tumor tissues with viral loads under or at the limit of quantification. New extraction from the same tumor blocks for these samples revealed no HPV with qPCR and INNO-Lipa assays. CONCLUSION: With adequate procedures and reliable techniques, no HPV was detected in the largest case-control study so far, bringing more evidence on the absence of involvement of HPV in CRCs.
Authors: Larisse Silva Dalla Libera; Thalita de Siqueira; Igor Lopes Santos; Jéssica Enocencio Porto Ramos; Amanda Xavier Milhomen; Rita de Cassia Gonçalves de Alencar; Silvia Helena Rabelo Santos; Megmar Aparecida Dos Santos Carneiro; Rosane Ribeiro Figueiredo Alves; Vera Aparecida Saddi Journal: PLoS One Date: 2020-06-25 Impact factor: 3.240