Literature DB >> 2792769

RepB' is required in trans for the two single-strand DNA initiation signals in oriV of plasmid RSF1010.

Y Honda1, H Sakai, T Komano, M Bagdasarian.   

Abstract

We have shown previously [Honda et al., Gene 68 (1988) 221-228] that the oriV region of the broad-host-range plasmid RSF1010 contained two single-strand DNA initiation signals (ssi) which have RSF1010-specific properties since they required one or more factors provided in trans by RSF1010 for their functional activities. We demonstrate here, by deletion analysis, that repB', one of the genes essential for the vegetative replication of RSF1010, produces a factor required for the function of ssi signals. It is conceivable that the RepB' protein and the two ssi signals, ssiA and ssiB, cooperatively compose plasmid-specific priming complexes which confer the broad-host-range property upon RSF1010.

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Year:  1989        PMID： 2792769     DOI： 10.1016/0378-1119(89)90261-8

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


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8.  A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010.

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