Ni Wayan Kurniani Karja1, Mokhamad Fahrudin1, Mohamad Agus Setiadi1, Ligaya Ita Tumbelaka2, Retno Sudarwati3, Yohana Tri Hastuti3, Bongot Huas Mulia3, Ardyta Widianti3, Keni Sultan3, Tsukasa Terazono4, Zhao Namula4, Masayasu Taniguchi4, Fuminori Tanihara5, Tatsuya Takemoto6, Kazuhiro Kikuchi7, Yoko Sato8, Takeshige Otoi9. 1. Faculty of Veterinary Science, Bogor Agricultural University, Indonesia. 2. Faculty of Veterinary Science, Bogor Agricultural University; Taman Safari Indonesia, Bogor, Indonesia. 3. Taman Safari Indonesia, Bogor, Indonesia. 4. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. 5. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi,; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan. 6. Fujii Memorial Institute of Medical Sciences, Tokushima University, Tokushima, Japan. 7. Department of Animal Science, National Institute of Agrobiological Sciences, Ibaraki, Japan. 8. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. pxx13340@nifty.ne.jp. 9. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
Abstract
BACKGROUND: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers. OBJECTIVE: This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae). MATERIALS AND METHODS: The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes. RESULTS: All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa. CONCLUSION: Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.
BACKGROUND: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers. OBJECTIVE: This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae). MATERIALS AND METHODS: The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes. RESULTS: All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa. CONCLUSION: Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.