K O Kalluf1, L N Arend2, T E Wuicik1, M Pilonetto1, F F Tuon3. 1. Department of Microbiology, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil. 2. Bacteriology Section, Laboratório Central de Saúde Pública do Estado LACEN, PR, Brazil. 3. Department of Medicine, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil; Division of Infectious Diseases, Universidade Federal do Paraná, Curitiba, PR, Brazil. Electronic address: flptuon@gmail.com.
Abstract
BACKGROUND: Infections caused by multidrug resistant microorganisms are a global health problem, and Pseudomonas aeruginosa is an important nosocomial pathogen, easily disseminated in the hospital environment. The aim of this study was to determine SPM-1 in P. aeruginosa strains in 30 Brazilian hospitals and the genetic similarity of isolates. METHODS: We analyzed 161 isolates of carbapenem-resistant P. aeruginosa. Imipenem/EDTA and imipenem strip were used for phenotypic detection of MBL production; and real-time polymerase chain reaction (PCR) for genetic detection. Genetic similarity was determined by rep-PCR. RESULTS: We obtained 136/161 (84.5%) isolates with positive phenotypic result for metallo-β-lactamase (MBL) and the blaSPM-1 gene was identified in 41 isolates. There was a predominant profile (>95% of genetic similarity) in 92.7% of isolates. This predominant profile was widely disseminated in Paraná state. CONCLUSION: SPM-1 is the main MBL identified in carbapenem-resistant P. aeruginosa in Southern Brazil. The genetic similarity among some isolates suggests a clonal expansion.
BACKGROUND: Infections caused by multidrug resistant microorganisms are a global health problem, and Pseudomonas aeruginosa is an important nosocomial pathogen, easily disseminated in the hospital environment. The aim of this study was to determine SPM-1 in P. aeruginosa strains in 30 Brazilian hospitals and the genetic similarity of isolates. METHODS: We analyzed 161 isolates of carbapenem-resistant P. aeruginosa. Imipenem/EDTA and imipenem strip were used for phenotypic detection of MBL production; and real-time polymerase chain reaction (PCR) for genetic detection. Genetic similarity was determined by rep-PCR. RESULTS: We obtained 136/161 (84.5%) isolates with positive phenotypic result for metallo-β-lactamase (MBL) and the blaSPM-1 gene was identified in 41 isolates. There was a predominant profile (>95% of genetic similarity) in 92.7% of isolates. This predominant profile was widely disseminated in Paraná state. CONCLUSION: SPM-1 is the main MBL identified in carbapenem-resistant P. aeruginosa in Southern Brazil. The genetic similarity among some isolates suggests a clonal expansion.
Authors: Marcelo Pillonetto; Regiane Tigulini de Souza Jordão; Gabriel Savogin Andraus; Ricardo Bergamo; Fabiano Barreto Rocha; Mayara Caroline Onishi; Bernardo Montesanti Machado de Almeida; Keite da Silva Nogueira; Amanda Dal Lin; Viviane Maria de Carvalho Hessel Dias; André Luiz de Abreu Journal: Front Public Health Date: 2021-01-14
Authors: Viviane Maria de Carvalho Hessel Dias; Daniela Maria Waszak da Silva; Marion Burger; Alcides Augusto Souto de Oliveira; Patrícia de Jesus Capelo; Fabio Augusto da Rocha Specian; Marianna Cavina de Figueiredo; Felipe Francisco Tuon; Cristina Pellegrino Baena Journal: Braz J Infect Dis Date: 2021-02-13 Impact factor: 3.257