Literature DB >> 27923522

Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing.

Bernard J Wolff1, Alvaro J Benitez1, Heta P Desai1, Shatavia S Morrison1, Maureen H Diaz1, Jonas M Winchell2.   

Abstract

We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations. Published by Elsevier Inc.

Entities:  

Keywords:  M. pneumoniae; Next generation sequencing; P1 typing; Real-time PCR

Mesh：

Substances：

Year:  2016        PMID： 27923522     DOI： 10.1016/j.diagmicrobio.2016.11.013

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

1.  Molecular Characterization of Mycoplasma pneumoniae Infections in Two Rural Populations of Thailand from 2009 to 2012.

Authors:  Toni Whistler; Pongpun Sawatwong; Maureen H Diaz; Alvaro J Benitez; Bernard J Wolff; Patranuch Sapchookul; Somsak Thamthitiwat; Jonas M Winchell
Journal:  J Clin Microbiol       Date:  2017-05-10       Impact factor: 5.948

2.  Comprehensive bioinformatics analysis of Mycoplasma pneumoniae genomes to investigate underlying population structure and type-specific determinants.

Authors:  Maureen H Diaz; Heta P Desai; Shatavia S Morrison; Alvaro J Benitez; Bernard J Wolff; Jason Caravas; Timothy D Read; Deborah Dean; Jonas M Winchell
Journal:  PLoS One       Date:  2017-04-14       Impact factor: 3.240

Review 3.  Advanced Role of Neutrophils in Common Respiratory Diseases.

Authors:  Jinping Liu; Zhiqiang Pang; Guoqiang Wang; Xuewa Guan; Keyong Fang; Ziyan Wang; Fang Wang
Journal:  J Immunol Res       Date:  2017-05-15       Impact factor: 4.818

4.  Mycoplasma pneumoniae outbreak, Southeastern Finland, 2017-2018: molecular epidemiology and laboratory diagnostic lessons.

Authors:  Satu Kurkela; Mirja Puolakkainen; Kati Hokynar; Tea Nieminen; Harri Saxen; Laura Mannonen; Risto Pietikäinen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-07-01       Impact factor: 3.267

5.  Effect Evaluation of Artificial Intelligence-Based Electronic Health PDCA Nursing Model in the Treatment of Mycoplasma Pneumonia in Children.

Authors:  Yan Zhao
Journal:  J Healthc Eng       Date:  2022-03-11       Impact factor: 2.682
  5 in total

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