Danica Zmejkoski1, Branka Petković2, Sofija Pavković-Lučić3, Zlatko Prolić4, Marko Anđelković3,5,6, Tatjana Savić6. 1. a Laboratory of Materials Science, University of Belgrade, Vinča Institute of Nuclear Sciences , Belgrade , Serbia. 2. b Department of Neurophysiology, University of Belgrade, Institute for Biological Research , Belgrade , Serbia. 3. c Chair of Genetics and Evolution, Faculty of Biology , University of Belgrade , Belgrade , Serbia. 4. d Department of Insect Physiology and Biochemistry , University of Belgrade, Institute for Biological Research , Belgrade , Serbia. 5. e Department of Chemical and Biological Sciences , Serbian Academy of Sciences and Arts , Belgrade , Serbia. 6. f Department of Genetics of Populations and Ecogenotoxicology , University of Belgrade, Institute for Biological Research , Belgrade , Serbia.
Abstract
PURPOSE: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines. MATERIALS AND METHODS: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50 Hz, 0.5 mT, 48 h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively. RESULTS: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males. CONCLUSIONS: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.
PURPOSE: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines. MATERIALS AND METHODS: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50 Hz, 0.5 mT, 48 h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively. RESULTS: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males. CONCLUSIONS: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.
Authors: Sebastian Shepherd; Georgina Hollands; Victoria C Godley; Suleiman M Sharkh; Chris W Jackson; Philip L Newland Journal: PLoS One Date: 2019-10-10 Impact factor: 3.240