C Virgiliou1, L Valianou1, M Witting2,3, F Moritz2, C Fotakis4, P Zoumpoulakis4, A C Chatziioannou1, L Lazaros5, G Makrydimas5, K Chatzimeletiou6, N Raikos7, G A Theodoridis1. 1. Department of Chemistry, Aristotle University, Thessaloniki, Greece. 2. Research Unit Analytical BioGeoChemistry Helmholtz Zentrum Munchen, German, Research Center for Health and Environment Neuherberg, Germany. 3. Chair of Analytical Foodchemistry, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzunng und Umwelt, Technische Universität München. 4. Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, Greece. 5. Department of Obstetrics & Gynecology, Ioannina University Hospital, Ioannina, Greece. 6. Unit for Human Reproduction, 1st Department of Obstetrics & Gynecology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece. 7. Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Abstract
AIM: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. METHODOLOGY: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied. Holistic analysis performed by GC-MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT-ICR-MS) instrumentation. RESULTS & CONCLUSIONS: Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies.
AIM: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. METHODOLOGY: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied. Holistic analysis performed by GC-MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT-ICR-MS) instrumentation. RESULTS & CONCLUSIONS: Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies.