Lida Sadeghinejad1, Dennis G Cvitkovitch1, Walter L Siqueira2, Justin Merritt3, J Paul Santerre1, Yoav Finer4. 1. Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada. 2. Schulich Dentistry and Department of Biochemistry, University of Western Ontario, London, Ontario, Canada. 3. Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA. 4. Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada. Electronic address: yoav.finer@dentistry.utoronto.ca.
Abstract
OBJECTIVES: Investigate the effects of a Bis-phenyl-glycidyl-dimethacrylate (BisGMA) biodegradation product, bishydroxypropoxyphenyl-propane (BisHPPP), on gene expression and protein synthesis of cariogenic bacteria. METHODS: Quantitative real-time polymerase chain reaction was used to investigate the effects of BisHPPP on the expression of specific virulence-associated genes, i.e. gtfB, gtfC, gbpB, comC, comD, comE and atpH in Streptococcus mutans UA159. Possible mechanisms for bacterial response to BisHPPP were explored using gene knock-out and associated complemented strains of the signal peptide encoding gene, comC. The effects of BisHPPP on global gene and protein expression was analyzed using microarray and quantitative proteomics. The role of BisHPPP in glucosyltransferase (GTF) enzyme activity of S. mutans biofilms was also measured. RESULTS: BisHPPP (0.01, 0.1mM) up-regulated gtfB/C, gbpB, comCDE, and atpH most pronounced in biofilms at cariogenic pH (5.5). The effects of BisHPPP on the constructed knock-out and complemented strains of comC from quorum-sensing system, implicated this signaling pathway in up-regulation of the virulence-associated genes. Microarray and proteomics identified BisHPPP-regulated genes and proteins involved in biofilm formation, carbohydrate transport, acid tolerance and stress-response. GTF activity was higher in BisHPPP-exposed biofilms when compared to no-BisHPPP conditions. SIGNIFICANCE: These findings provide insight into the genetic and physiological pathways and mechanisms that help explain S. mutans adaptation to restorative conditions that are conducive to increased secondary caries around resin composite restorations and may provide guidance to clinicians' decision on the selection of dental materials when considering the long term oral health of patients and the interactions of composite resins with oral bacteria.
OBJECTIVES: Investigate the effects of a Bis-phenyl-glycidyl-dimethacrylate (BisGMA) biodegradation product, bishydroxypropoxyphenyl-propane (BisHPPP), on gene expression and protein synthesis of cariogenic bacteria. METHODS: Quantitative real-time polymerase chain reaction was used to investigate the effects of BisHPPP on the expression of specific virulence-associated genes, i.e. gtfB, gtfC, gbpB, comC, comD, comE and atpH in Streptococcus mutans UA159. Possible mechanisms for bacterial response to BisHPPP were explored using gene knock-out and associated complemented strains of the signal peptide encoding gene, comC. The effects of BisHPPP on global gene and protein expression was analyzed using microarray and quantitative proteomics. The role of BisHPPP in glucosyltransferase (GTF) enzyme activity of S. mutans biofilms was also measured. RESULTS:BisHPPP (0.01, 0.1mM) up-regulated gtfB/C, gbpB, comCDE, and atpH most pronounced in biofilms at cariogenic pH (5.5). The effects of BisHPPP on the constructed knock-out and complemented strains of comC from quorum-sensing system, implicated this signaling pathway in up-regulation of the virulence-associated genes. Microarray and proteomics identified BisHPPP-regulated genes and proteins involved in biofilm formation, carbohydrate transport, acid tolerance and stress-response. GTF activity was higher in BisHPPP-exposed biofilms when compared to no-BisHPPP conditions. SIGNIFICANCE: These findings provide insight into the genetic and physiological pathways and mechanisms that help explain S. mutans adaptation to restorative conditions that are conducive to increased secondary caries around resin composite restorations and may provide guidance to clinicians' decision on the selection of dental materials when considering the long term oral health of patients and the interactions of composite resins with oral bacteria.
Authors: Peter C Y Lau; Chang Kyoo Sung; Janet H Lee; Donald A Morrison; Dennis G Cvitkovitch Journal: J Microbiol Methods Date: 2002-04 Impact factor: 2.363
Authors: Yung-Hua Li; Peter C Y Lau; Nan Tang; Gunnel Svensäter; Richard P Ellen; Dennis G Cvitkovitch Journal: J Bacteriol Date: 2002-11 Impact factor: 3.490
Authors: Bo Huang; Lida Sadeghinejad; Olabisi I A Adebayo; Dengbo Ma; Yizhi Xiao; Walter L Siqueira; Dennis G Cvitkovitch; Yoav Finer Journal: Acta Biomater Date: 2018-09-28 Impact factor: 8.947
Authors: L M Barcelos; M G Borges; C J Soares; M S Menezes; V Huynh; M G Logan; A P P Fugolin; C S Pfeifer Journal: Dent Mater Date: 2020-01-28 Impact factor: 5.304
Authors: Ana P Fugolin; Andreia B de Paula; Adam Dobson; Vincent Huynh; Rafael Consani; Jack L Ferracane; Carmem S Pfeifer Journal: Dent Mater Date: 2020-05-08 Impact factor: 5.304