Literature DB >> 27918140

Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water.

Dennis Kurzbach1,2, Estel Canet1,2, Andrea G Flamm3, Aditya Jhajharia1,2, Emmanuelle M M Weber1,2, Robert Konrat3, Geoffrey Bodenhausen1,2.   

Abstract

Hyperpolarized water can selectively enhance NMR signals of rapidly exchanging protons in osteopontin (OPN), a metastasis-associated intrinsically disordered protein (IDP), at near-physiological pH and temperature. The transfer of magnetization from hyperpolarized water is limited to solvent-exposed residues and therefore selectively enhances signals in 1 H-15 N correlation spectra. Binding to the polysaccharide heparin was found to induce the unfolding of preformed structural elements in OPN.
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  NMR spectroscopy; dynamic nuclear polarization; hyperpolarized water; intrinsically disordered proteins; solvent exchange

Mesh：

Substances：

Year:  2016        PMID： 27918140     DOI： 10.1002/anie.201608903

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  15 in total

1.  Laser- and cryogenic probe-assisted NMR enables hypersensitive analysis of biomolecules at submicromolar concentration.

Authors:  Yusuke Okuno; Miranda F Mecha; Hanming Yang; Lingchao Zhu; Charles G Fry; Silvia Cavagnero
Journal:  Proc Natl Acad Sci U S A       Date:  2019-05-29       Impact factor: 11.205

2.  Real-Time Analysis of Folding upon Binding of a Disordered Protein by Using Dissolution DNP NMR Spectroscopy.

Authors:  Mukundan Ragavan; Luigi I Iconaru; Cheon-Gil Park; Richard W Kriwacki; Christian Hilty
Journal:  Angew Chem Int Ed Engl       Date:  2017-05-16       Impact factor: 15.336

3.  Detection of Protein-Ligand Interactions by 19F Nuclear Magnetic Resonance Using Hyperpolarized Water.

Authors:  Jiandu Hu; Jihyun Kim; Christian Hilty
Journal:  J Phys Chem Lett       Date:  2022-04-24       Impact factor: 6.888

Review 4.  Hyperpolarized water as universal sensitivity booster in biomolecular NMR.

Authors:  Christian Hilty; Dennis Kurzbach; Lucio Frydman
Journal:  Nat Protoc       Date:  2022-05-11       Impact factor: 17.021

5.  A 300-fold enhancement of imino nucleic acid resonances by hyperpolarized water provides a new window for probing RNA refolding by 1D and 2D NMR.

Authors:  Mihajlo Novakovic; Gregory L Olsen; György Pintér; Daniel Hymon; Boris Fürtig; Harald Schwalbe; Lucio Frydman
Journal:  Proc Natl Acad Sci U S A       Date:  2020-01-16       Impact factor: 11.205

Review 6.  Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts.

Authors:  Sami Jannin; Jean-Nicolas Dumez; Patrick Giraudeau; Dennis Kurzbach
Journal:  J Magn Reson       Date:  2019-06-04       Impact factor: 2.229

Review 7.  13C Direct Detected NMR for Challenging Systems.

Authors:  Isabella C Felli; Roberta Pierattelli
Journal:  Chem Rev       Date:  2022-01-13       Impact factor: 72.087

8.  Structural Insights into the Osteopontin-Aptamer Complex by Molecular Dynamics Simulations.

Authors:  Giovanni La Penna; Riccardo Chelli
Journal:  Front Chem       Date:  2018-01-30       Impact factor: 5.221

9.  Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44.

Authors:  Gulimirerouzi Fnu; Palak Agrawal; Gopal C Kundu; Georg F Weber
Journal:  Biomolecules       Date:  2021-05-30

10.  Impact of the Hereditary P301L Mutation on the Correlated Conformational Dynamics of Human Tau Protein Revealed by the Paramagnetic Relaxation Enhancement NMR Experiments.

Authors:  Ryosuke Kawasaki; Shin-Ichi Tate
Journal:  Int J Mol Sci       Date:  2020-05-30       Impact factor: 5.923
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.