| Literature DB >> 27914079 |
Juan J Quereda1,2,3, Martin Sachse4, Damien Balestrino1,2,3,5, Théodore Grenier1,2,3, Jennifer Fredlund6, Anne Danckaert7, Nathalie Aulner7, Spencer Shorte7, Jost Enninga6, Pascale Cossart1,2,3, Javier Pizarro-Cerdá8,9,10.
Abstract
Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.Entities:
Keywords: CCF4; Correlative light/electron microcopy (CLEM); Förster resonance energy transfer (FRET) microscopy; Listeria monocytogenes; Listeriolysin O; Phagocytosis; Phospholipases; Vacuole
Mesh：
Year: 2017 PMID： 27914079 DOI： 10.1007/978-1-4939-6673-8_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745