| Literature DB >> 27914008 |
Hsiao-Yu Chiu1,2, Chen-Huan Lin3, Chung Y Hsu4, John Yu5,6, Chia-Hung Hsieh7, Woei-Cherng Shyu8,9.
Abstract
Until now, the surface markers of multipotent mesenchymal stem cells (MSCs) had not been fully identified. Here, we found that the IGF1 receptor (IGF1R), regarded as a pluripotent marker of embryonic stem cells (ESCs), was also expressed in human dental pulp derived-mesenchymal stem cells (hDSCs), which displayed a potential for both self-renewal and multipotency. hDSC-secreted IGF1 interacted with IGF1R through an autocrine signaling pathway to maintain this self-renewal and proliferation potential. Stereotaxic implantation of immunosorted IGF1R+ hDSCs in rats with neonatal hypoxia-ischemia (NHI) promoted neuroplasticity, improving the neurological outcome by increasing expression of the anti-apoptotic protein Bcl-2, which enhanced both neurogenesis and angiogenesis. In addition, treatment with IGF1R+ hDSCs significantly modulated neurite regeneration and anti-inflammation in vivo in NHI rats and in vitro in primary cortical cultures under oxygen/glucose deprivation. Autocrine regulatory expression of IGF1R contributed to maintaining the self-renewal capacity of hDSCs. Furthermore, implantation of IGF1R+ hDSCs increased neuroplasticity with neurite regeneration and immunomodulation in and the NHI rat model.Entities:
Keywords: Human dental pulp mesenchymal stem cells (hDSCs); Insulin-like growth factor 1 (IGF1); Insulin-like growth factor 1 receptor (IGF1R); Neonatal hypoxia-ischemia model; Neuroplasticity
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Year: 2016 PMID： 27914008 DOI： 10.1007/s12035-016-0210-y
Source DB: PubMed Journal: Mol Neurobiol ISSN： 0893-7648 Impact factor: 5.590