Literature DB >> 27910049

Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells.

Francesca Stillitano1, Ioannis Karakikes2,3, Roger J Hajjar4.   

Abstract

The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies.The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. Then, we provide a basic procedure to transduce hiPSC-CMs.

Entities:  

Keywords:  Cardiac differentiation; Gene therapy; Human pluripotent stem cells (hPSCs); Transduction; hPSC-derived cardiomyocytes

Mesh：

Year:  2017        PMID： 27910049     DOI： 10.1007/978-1-4939-6588-5_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  The Gene Therapy Resource Program: A Decade of Dedication to Translational Research by the National Heart, Lung, and Blood Institute.

Authors:  Terence R Flotte; Eric Daniels; Janet Benson; Jeneé M Bevett-Rose; Kenneth Cornetta; Margaret Diggins; Julie Johnston; Susan Sepelak; Johannes C M van der Loo; James M Wilson; Cheryl L McDonald
Journal:  Hum Gene Ther Clin Dev       Date:  2017-11-27       Impact factor: 5.032

2.  Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles.

Authors:  Megan A Yamoah; Maryam Moshref; Janhavi Sharma; Wei Chun Chen; Hannah A Ledford; Jeong Han Lee; Karen S Chavez; Wenying Wang; Javier E López; Deborah K Lieu; Padmini Sirish; Xiao-Dong Zhang
Journal:  Int J Nanomedicine       Date:  2018-10-05
  2 in total

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