Jianghao Chen1, Wei Liu1, Jinxiu Zhao2, Cong Sun2, Jie Chen1, Kaijin Hu1, Linlin Zhang1, Yuxiang Ding3. 1. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center of Oral Diseases, Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University, 145 Western Changle Road, Xi'an, 710032, People's Republic of China. 2. Department of Stomatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China. 3. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center of Oral Diseases, Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University, 145 Western Changle Road, Xi'an, 710032, People's Republic of China. yxding@fmmu.edu.cn.
Abstract
OBJECTIVES: To investigate the therapeutic effect of gelatin microspheres containing different concentrations of calcitonin gene-related peptide (CGRP) or substance P on repairing bone defects in a rabbit osteoporosis model. RESULTS: Gelatin microspheres containing different concentrations of CGRP or substance P promoted osteogenesis after 3 months in a rabbit osteoporotic bone defective model. From micro-computed tomography imaging results, 10 nM CGRP was optimal for increasing the trabecular number and decreasing the trabecular bone separation degree; similar effects were observed with the microspheres containing 1 µM substance P. Histological analysis showed that the gelatin microspheres containing CGRP or substance P, regardless of the concentration, effectively promoted osteogenesis, and the highest effect was achieved in the groups containing 1 µM CGRP or 1 µM substance P. CONCLUSIONS: Gelatin microspheres containing CGRP or substance P effectively promoted osteogenesis in a rabbit osteoporotic bone defect model dose-dependently, though their effects in repairing human alveolar ridge defects still need further investigation.
OBJECTIVES: To investigate the therapeutic effect of gelatin microspheres containing different concentrations of calcitonin gene-related peptide (CGRP) or substance P on repairing bone defects in a rabbit osteoporosis model. RESULTS: Gelatin microspheres containing different concentrations of CGRP or substance P promoted osteogenesis after 3 months in a rabbit osteoporotic bone defective model. From micro-computed tomography imaging results, 10 nM CGRP was optimal for increasing the trabecular number and decreasing the trabecular bone separation degree; similar effects were observed with the microspheres containing 1 µM substance P. Histological analysis showed that the gelatin microspheres containing CGRP or substance P, regardless of the concentration, effectively promoted osteogenesis, and the highest effect was achieved in the groups containing 1 µM CGRP or 1 µM substance P. CONCLUSIONS: Gelatin microspheres containing CGRP or substance P effectively promoted osteogenesis in a rabbit osteoporotic bone defect model dose-dependently, though their effects in repairing humanalveolar ridge defects still need further investigation.
Entities:
Keywords:
Bone defect; Bone repair; Calcitonin gene-related peptide; Gelatin microspheres; Osteoporosis; Substance P
Authors: Seungyup Sun; Nicklaus H Diggins; Zachary J Gunderson; Jill C Fehrenbacher; Fletcher A White; Melissa A Kacena Journal: Bone Date: 2019-11-09 Impact factor: 4.398
Authors: María Permuy; Mónica López-Peña; Fernando Muñoz; Antonio González-Cantalapiedra Journal: J Bone Miner Metab Date: 2019-05-13 Impact factor: 2.626