Literature DB >> 2790022

Characterization of glycosphingolipid mixtures with up to ten sugars by gas chromatography and gas chromatography-mass spectrometry as permethylated oligosaccharides and ceramides released by ceramide glycanase.

G C Hansson1, Y T Li, H Karlsson.   

Abstract

A novel, effective method for structural characterization of glycosphingolipids has been devised. It employs ceramide glycanase to release intact oligosaccharides followed by analysis using high-mass gas chromatography-mass spectrometry. The oligosaccharides and ceramides released by the glycanase were permethylated and analyzed. The capillary gas chromatography gave excellent resolution and separated, for example, two isomeric 10-sugar oligosaccharides with a molecular mass of 2150 daltons differing only by a Gal1-3GlcNAc and a Gal1-4GlcNAc linkage. The oligosaccharides released from sialic acid containing glycosphingolipids (gangliosides) were also analyzed for monosialo compounds. This analytical approach is simple, is quick, and can readily allow quantitation of individual glycosphingolipids.

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Year:  1989        PMID： 2790022     DOI： 10.1021/bi00442a021

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  8 in total

1.  Monoclonal antibody GOM-2 binds to blood group B-Le(y) active glycolipid antigens on human gastric cancer cells, KATO-III.

Authors:  S Sueyoshi; H Nagakura; A Kato; S Uetsuki; Y Nakayama; M Adachi
Journal:  Glycoconj J       Date:  1992-04       Impact factor: 2.916

2.  Structure determination of ceramides and neutral glycosphingolipids by collisional activation of [M + Li](+) ions.

Authors:  Q Ann; J Adams
Journal:  J Am Soc Mass Spectrom       Date:  1992-03       Impact factor: 3.109

3.  Liquid chromatography "on-flow" 1H nuclear magnetic resonance on native glycosphingolipid mixtures together with gas chromatography/mass spectrometry on the released oligosaccharides for screening and characterisation of carbohydrate-based antigens from pig lungs.

Authors:  A E Bäcker; S Thorbert; O Rakotonirainy; E C Hallberg; A Olling; M Gustavsson; B E Samuelsson; B Soussi
Journal:  Glycoconj J       Date:  1999-01       Impact factor: 2.916

4.  Ceramide glycanase from the earthworm, Lumbricus terrestris.

Authors:  B Z Carter; S C Li; Y T Li
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

5.  Polysialic acid engineering: synthesis of polysialylated neoglycosphingolipids by using the polysialyltransferase from neuroinvasive Escherichia coli K1.

Authors:  J W Cho; F A Troy
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-22       Impact factor: 11.205

6.  High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides.

Authors:  H Karlsson; N Karlsson; G C Hansson
Journal:  Mol Biotechnol       Date:  1994-04       Impact factor: 2.695

7.  Analysis of glycosphingolipid-derived oligosaccharides by high pH anion exchange chromatography.

Authors:  S Ruan; K O Lloyd
Journal:  Glycoconj J       Date:  1994-06       Impact factor: 2.916

8.  The diversity of O-linked glycans expressed during Drosophila melanogaster development reflects stage- and tissue-specific requirements for cell signaling.

Authors:  Kazuhiro Aoki; Mindy Porterfield; Samuel S Lee; Brian Dong; Khoi Nguyen; Katherine H McGlamry; Michael Tiemeyer
Journal:  J Biol Chem       Date:  2008-08-25       Impact factor: 5.157
  8 in total

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