Sandra Dolz1, David Górriz2, José Ignacio Tembl2, Dolors Sánchez2, Gerardo Fortea2, Vera Parkhutik2, Aida Lago2. 1. From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.). sandolzgi@gmail.com. 2. From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
Abstract
BACKGROUND AND PURPOSE: Progression of asymptomatic carotid artery stenosis (ACAS) in patients with >50% luminal narrowing is considered a potential risk factor for ischemic stroke; however, subclinical molecular biomarkers of ACAS progression are lacking. Recent studies suggest a regulatory function for several microRNAs (miRNAs) on the evolution of carotid plaque, but its role in ACAS progression is mostly unknown. The aim of our study was to investigate a wide miRNA panel in peripheral blood exosomes from patients with ACAS to associate circulating miRNA expression profiles with stenosis progression. METHODS: The study included 60 patients with ACAS carrying >50% luminal narrowing. First, miRNA expression profiles of circulating exosomes were determined by Affymetrix microarrays from plasma samples of 16 patients from the cohort. Second, those miRNAs among the most differentially expressed in patients with ACAS progression were quantified by real-time polymerase chain reaction in a separate replication cohort of 39 subjects within the patient sample. RESULTS: Our results showed that ACAS progression was associated with development of stroke. MiR-199b-3p, miR-27b-3p, miR-130a-3p, miR-221-3p, and miR-24-3p presented significant higher expression in those patients with ACAS progression. CONCLUSIONS: In conclusion, our study supports that specific circulating miRNA expression profiles could provide a new tool that complements the monitoring of ACAS progression, improving therapeutic approaches to prevent ischemic stroke.
BACKGROUND AND PURPOSE: Progression of asymptomatic carotid artery stenosis (ACAS) in patients with >50% luminal narrowing is considered a potential risk factor for ischemic stroke; however, subclinical molecular biomarkers of ACAS progression are lacking. Recent studies suggest a regulatory function for several microRNAs (miRNAs) on the evolution of carotid plaque, but its role in ACAS progression is mostly unknown. The aim of our study was to investigate a wide miRNA panel in peripheral blood exosomes from patients with ACAS to associate circulating miRNA expression profiles with stenosis progression. METHODS: The study included 60 patients with ACAS carrying >50% luminal narrowing. First, miRNA expression profiles of circulating exosomes were determined by Affymetrix microarrays from plasma samples of 16 patients from the cohort. Second, those miRNAs among the most differentially expressed in patients with ACAS progression were quantified by real-time polymerase chain reaction in a separate replication cohort of 39 subjects within the patient sample. RESULTS: Our results showed that ACAS progression was associated with development of stroke. MiR-199b-3p, miR-27b-3p, miR-130a-3p, miR-221-3p, and miR-24-3p presented significant higher expression in those patients with ACAS progression. CONCLUSIONS: In conclusion, our study supports that specific circulating miRNA expression profiles could provide a new tool that complements the monitoring of ACAS progression, improving therapeutic approaches to prevent ischemic stroke.
Authors: Zheng Qin; Ruoxi Liao; Yuqin Xiong; Luojia Jiang; Jiameng Li; Liya Wang; Mei Han; Si Sun; Jiwen Geng; Qinbo Yang; Zhuyun Zhang; Yupei Li; Heyue Du; Baihai Su Journal: Ann Transl Med Date: 2021-04
Authors: Laura Otero-Ortega; Fernando Laso-García; MariCarmen Gómez-de Frutos; Blanca Fuentes; Luke Diekhorst; Exuperio Díez-Tejedor; María Gutiérrez-Fernández Journal: Transl Stroke Res Date: 2018-08-13 Impact factor: 6.829