| Literature DB >> 27896757 |
Gustavo Henrique Martins Ferreira Souza1, Paul C Guest2, Daniel Martins-de-Souza2.
Abstract
Proteomic tools can only be implemented in clinical settings if high-throughput, automated, sensitive, and accurate methods are developed. This has driven researchers to the edge of mass spectrometry (MS)-based proteomics capacity. Here we provide an overview of recent achievements in mass spectrometric technologies and instruments. This includes development of high and ultra definition-MSE (HDMSE and UDMSE) through implementation of ion mobility (IM) MS towards sensitive and accurate label-free proteomics using ultra performance liquid chromatography (UPLC). Label free UPLC-HDMSE is less expensive than labeled-based quantitative proteomics and has no limits regarding the number of samples that can be analyzed and compared, which is an important requirement for supporting clinical applications.Keywords: DIA; Data independent acquisition; HDMSE; Label-free; MSE; Proteomics; UDMSE
Mesh：
Substances：
Year: 2017 PMID： 27896757 DOI： 10.1007/978-1-4939-6730-8_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745