| Literature DB >> 27896562 |
Dellane Martins Tigre1,2, Camila Fonseca Lopes Brandão3,4, Fabiana Lopes de Paula3,5, Fabio Alexandre Chinalia6, Gubio Soares Campos3, Silvia Ines Sardi3,6.
Abstract
Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.Entities:
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Year: 2016 PMID： 27896562 DOI： 10.1007/s00705-016-3172-5
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574