| Literature DB >> 27895202 |
Weiwei Ma1,2, Fuqing Wu1,2, Peike Sheng1,2, Xiaole Wang1,2, Zhe Zhang1,2, Kunneng Zhou1,2, Huan Zhang1,2, Jinlong Hu1,2, Qibin Lin1,2, Zhijun Cheng1,2, Jiulin Wang1,2, Shanshan Zhu1,2, Xin Zhang1,2, Xiuping Guo1,2, Haiyang Wang1,2, Chuanyin Wu1,2, Huqu Zhai1,2, Jianmin Wan3,4.
Abstract
The shoot apical meristem (SAM) consists of a population of multipotent cells that generates all aerial structures and regenerates itself. SAM maintenance and lateral organ development are regulated by several complex signaling pathways, in which the Argonaute gene-mediated pathway plays a key role. One Argonaute gene, AGO10, functions as a microRNA locker that attenuates miR165/166 activity and positively regulates shoot apical meristem development, but little is known about when and how AGO10 is regulated at the transcriptional level. In this work, we showed that transgenic rice plants overexpressing LBD12-1, an LBD family transcription factor, exhibited stunted growth, twisted leaves, abnormal anthers, and reduced SAM size. Further research revealed that LBD12-1 directly binds to the promoter region and represses the expression of AGO10. Overexpression of AGO10 in an LBD12-1 overexpression background rescued the growth defect phenotype of LBD12-1-overexpressing plants. The expression of LBD12-1 and its binding ability to the AGO10 promoter is induced by stress. lbd12-1 loss-of-function mutants showed similar phenotypes and SAM size to the wild type under normal conditions, but lbd12-1 had a larger SAM under salt stress. Our findings provide novel insights into the regulatory mechanism of AGO10 by which SAM size is controlled under stress conditions.Entities:
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Year: 2016 PMID： 27895202 PMCID： PMC5210715 DOI： 10.1104/pp.16.01699
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340