Shunnichi Saito1, Kenneth E Nollet1,2, Alain M Ngoma3, Takako Ono1, Hitoshi Ohto1,2. 1. Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan. 2. Radiation Medical Science Center, Fukushima Medical University, Fukushima, Japan. 3. Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, Quebec, Canada.
Abstract
BACKGROUND: Storage lesion, including microparticle formation, has been partially characterised in whole blood, but not in all combinations of pre-storage leucofiltration and/or irradiation. MATERIALS AND METHODS: Single-donor whole blood products were processed into four subunits: with and without leucofiltration, with and without X-irradiation (25 Gy). Platelet-, leucocyte-, and erythrocyte-derived microparticles and free haemoglobin were measured periodically throughout 42 days of storage. RESULTS: Pre-storage leucofiltration substantially reduced platelet- and leucocyte-derived microparticle counts throughout storage. Irradiation, in contrast, had no significant effect on microparticle counts. A gate for all microparticles showed a substantial time-dependent increase in unfiltered whole blood. A time-dependent increase in free haemoglobin was greatest in unfiltered, irradiated whole blood. DISCUSSION: This study indicates that leucofiltration can prevent the formation of leucocyte- and platelet-derived microparticles, and might reduce haemolysis in irradiated whole blood, either by removing factors that provoke haemolysis, or by selective retention of senescent or effete red cells most prone to haemolysis.
BACKGROUND: Storage lesion, including microparticle formation, has been partially characterised in whole blood, but not in all combinations of pre-storage leucofiltration and/or irradiation. MATERIALS AND METHODS: Single-donor whole blood products were processed into four subunits: with and without leucofiltration, with and without X-irradiation (25 Gy). Platelet-, leucocyte-, and erythrocyte-derived microparticles and free haemoglobin were measured periodically throughout 42 days of storage. RESULTS: Pre-storage leucofiltration substantially reduced platelet- and leucocyte-derived microparticle counts throughout storage. Irradiation, in contrast, had no significant effect on microparticle counts. A gate for all microparticles showed a substantial time-dependent increase in unfiltered whole blood. A time-dependent increase in free haemoglobin was greatest in unfiltered, irradiated whole blood. DISCUSSION: This study indicates that leucofiltration can prevent the formation of leucocyte- and platelet-derived microparticles, and might reduce haemolysis in irradiated whole blood, either by removing factors that provoke haemolysis, or by selective retention of senescent or effete red cells most prone to haemolysis.
Authors: F Alvarado-Ramy; M J Kuehnert; J Alonso-Echanove; L Sledge; N R Haley; J Epstein; J Vostal; M Pearson Journal: Transfus Med Date: 2006-02 Impact factor: 2.019
Authors: Mark Bates; Sarah Watts; Heidi Doughty; Tom Woolley; Andrew Miles; Liam Barry; Dominic Jenner; Andrew Sedman; Robert Purcell; Emrys Kirkman Journal: Transfusion Date: 2021-07 Impact factor: 3.337