Literature DB >> 27893304

Blame it on Southern, but it's a western blot.

Daniel J Klionsky1.   

Abstract

Edwin M. Southern is a professor emeritus at the University of Oxford. He is perhaps best known for development of the "Southern blot" (Dr. Southern was at the University of Edinburgh when he wrote his landmark paper). The Southern blot provided a scientific breakthrough by allowing scientists to detect a particular DNA sequence without first purifying it from the rest of the genome; the basic method involves the transfer of the DNA to a membrane, followed by detection with a specific probe. Although few people perform Southern blots as originally carried out by Southern, due in part to the more recent technique of the polymerase chain reaction, the basic concept continues to play an important role in molecular biology.

Entities:  

Keywords:  autophagy; lysosome; stress; vacuole; yeast

Mesh:

Year:  2017        PMID: 27893304      PMCID: PMC5240824          DOI: 10.1080/15548627.2016.1255382

Source DB:  PubMed          Journal:  Autophagy        ISSN: 1554-8627            Impact factor:   16.016


  8 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Identification of protein interactions by far Western analysis.

Authors:  D G Edmondson; S Y Roth
Journal:  Curr Protoc Mol Biol       Date:  2001-08

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

5.  A new blotting medium for the simple isolation and identification of highly resolved messenger RNA.

Authors:  D H Wreschner; M Herzberg
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

6.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

7.  The detection of DNA-binding proteins by protein blotting.

Authors:  B Bowen; J Steinberg; U K Laemmli; H Weintraub
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

8.  Enzyme-linked immunosorbent assay for glycyrrhizin using anti-glycyrrhizin monoclonal antibody and an eastern blotting technique for glucuronides of glycyrrhetic acid.

Authors:  S Shan; H Tanaka; Y Shoyama
Journal:  Anal Chem       Date:  2001-12-15       Impact factor: 6.986

  8 in total
  2 in total

1.  Inactivation of MTOR promotes autophagy-mediated epithelial injury in particulate matter-induced airway inflammation.

Authors:  Yin-Fang Wu; Zhou-Yang Li; Ling-Ling Dong; Wei-Jie Li; Yan-Ping Wu; Jing Wang; Hai-Pin Chen; Hui-Wen Liu; Miao Li; Ci-Liang Jin; Hua-Qiong Huang; Song-Min Ying; Wen Li; Hua-Hao Shen; Zhi-Hua Chen
Journal:  Autophagy       Date:  2019-06-16       Impact factor: 16.016

2.  Look youse guys and gals, dat just ain't right.

Authors:  Daniel J Klionsky
Journal:  Autophagy       Date:  2021-11-15       Impact factor: 13.391

  2 in total

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