Literature DB >> 2789038

A simple enzymic method to separate [3H]inositol 1,4,5- and 1,3,4-trisphosphate isomers in tissue extracts.

E D Kennedy1, I H Batty, E R Chilvers, S R Nahorski.   

Abstract

A novel method to separate [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4)P3 in tissue extracts is described. It is based on the selective metabolism of Ins(1,3,4)P3 by a crude cerebral supernatant in a Mg2+-free buffer followed by separation of [3H]inositol trisphosphates using conventional anion-exchange chromatography. Evaluation of the assay was performed using [3H]Ins(1,3,4)P3 standards and tissue extracts containing different proportions of [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4)P3. Parallel h.p.l.c. separations of extracts established the selective and complete metabolism of [3H]Ins(1,3,4)P3 under the above conditions and demonstrated that the enzymic method provides an accurate estimate of the trisphosphate isomers in rat cerebral cortex, parotid gland and bovine tracheal smooth muscle.

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Year:  1989        PMID： 2789038      PMCID： PMC1138658          DOI： 10.1042/bj2600283

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Similar effects of substance P and related peptides on salivation and on phosphatidylinositol turnover in rat salivary glands.

Authors:  M R Hanley; C M Lee; R H Michell; L M Jones
Journal:  Mol Pharmacol       Date:  1980-07       Impact factor: 4.436

2.  Pathway for inositol 1,3,4-trisphosphate and 1,4-bisphosphate metabolism.

Authors:  R C Inhorn; V S Bansal; P W Majerus
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

3.  Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.

Authors:  P T Hawkins; L Stephens; C P Downes
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

4.  The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues.

Authors:  R F Irvine; A J Letcher; J P Heslop; M J Berridge
Journal:  Nature       Date:  1986 Apr 17-23       Impact factor: 49.962

5.  Inositol phospholipid hydrolysis in rat cerebral cortical slices: I. Receptor characterisation.

Authors:  E Brown; D A Kendall; S R Nahorski
Journal:  J Neurochem       Date:  1984-05       Impact factor: 5.372

6.  Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.

Authors:  I R Batty; S R Nahorski; R F Irvine
Journal:  Biochem J       Date:  1985-11-15       Impact factor: 3.857

7.  Stepwise enzymatic dephosphorylation of inositol 1,4,5-trisphosphate to inositol in liver.

Authors:  D J Storey; S B Shears; C J Kirk; R H Michell
Journal:  Nature       Date:  1984 Nov 22-28       Impact factor: 49.962

8.  Accumulation of inositol polyphosphate isomers in agonist-stimulated cerebral-cortex slices. Comparison with metabolic profiles in cell-free preparations.

Authors:  I H Batty; A J Letcher; S R Nahorski
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

9.  Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands.

Authors:  R F Irvine; E E Anggård; A J Letcher; C P Downes
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

10.  Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.

Authors:  C P Downes; P T Hawkins; R F Irvine
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857
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  2 in total

1.  The effects of acute and chronic lithium treatment on pilocarpine-stimulated phosphoinositide hydrolysis in mouse brain in vivo.

Authors:  P Whitworth; D J Heal; D A Kendall
Journal:  Br J Pharmacol       Date:  1990-09       Impact factor: 8.739

2.  Determination of mass changes in phosphatidylinositol 4,5-bisphosphate and evidence for agonist-stimulated metabolism of inositol 1,4,5-trisphosphate in airway smooth muscle.

Authors:  E R Chilvers; I H Batty; R A Challiss; P J Barnes; S R Nahorski
Journal:  Biochem J       Date:  1991-04-15       Impact factor: 3.857
  2 in total

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