Ralf Hass1, Roland Jacobs2, Andreas M Kaufmann3, Peter Hillemanns4, Philipp Soergel5. 1. Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: Hass.Ralf@mh-hannover.de. 2. Department of Clinical Immunology and Rheumatology, Hannover Medical School, Hannover, Germany. Electronic address: Jacobs.Roland@mh-hannover.de. 3. Clinic for Gynecology, Charité-Universitätsmedizin, Berlin, Germany. Electronic address: Andreas.Kaufmann@charite.de. 4. Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: Hillemanns.Peter@mh-hannover.de. 5. Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: soergel.philipp@mh-hannover.de.
Abstract
BACKGROUND: Effects of photodynamic therapy (PDT) were tested with respect to immune cell stimulation in cervical intraepithelial neoplasia (CIN). METHODS: A patient with CIN received hexaminolevulinate (HAL) and subsequent PDT. These data were compared to a placebo PDT patient and a healthy HPV16-vaccinated donor. Isolation of peripheral blood mononuclear cells (PBMC) was performed before PDT and at 4 different time points after PDT. The proliferation of these PBMC was tested by [3H]thymidine incorporation following stimulation with control or HPV16-L1 peptides. Potential effects on the CD4+ and CD8+ cell subsets were analysed. RESULTS: The data revealed an unchanged or decreased proliferation of HPV16-L1 peptide-stimulated PBMC as compared to placebo patient. HPV16-L1 peptide incubation of PBMC from the HAL patient demonstrated significant proliferative capacity after PDT. The CD4+ and CD8+ T cell populations in placebo patient were slightly increased after HPV16-L1 peptide administration at each time point. Mixed results were obtained for CD4+ cells as compared to controls and nearly unchanged amounts of CD8+ cells were detectable following HPV16-L1 peptide exposure of PBMC from the HAL/PDT-treated patient. CONCLUSIONS: These findings suggest a T cell reaction from CIN patients during repeated HAL/PDT treatment. However, further immune cell populations might be involved during PDT.
BACKGROUND: Effects of photodynamic therapy (PDT) were tested with respect to immune cell stimulation in cervical intraepithelial neoplasia (CIN). METHODS: A patient with CIN received hexaminolevulinate (HAL) and subsequent PDT. These data were compared to a placebo PDT patient and a healthy HPV16-vaccinated donor. Isolation of peripheral blood mononuclear cells (PBMC) was performed before PDT and at 4 different time points after PDT. The proliferation of these PBMC was tested by [3H]thymidine incorporation following stimulation with control or HPV16-L1 peptides. Potential effects on the CD4+ and CD8+ cell subsets were analysed. RESULTS: The data revealed an unchanged or decreased proliferation of HPV16-L1 peptide-stimulated PBMC as compared to placebo patient. HPV16-L1 peptide incubation of PBMC from the HALpatient demonstrated significant proliferative capacity after PDT. The CD4+ and CD8+ T cell populations in placebo patient were slightly increased after HPV16-L1 peptide administration at each time point. Mixed results were obtained for CD4+ cells as compared to controls and nearly unchanged amounts of CD8+ cells were detectable following HPV16-L1 peptide exposure of PBMC from the HAL/PDT-treated patient. CONCLUSIONS: These findings suggest a T cell reaction from CINpatients during repeated HAL/PDT treatment. However, further immune cell populations might be involved during PDT.