Ibrahim Hammoud1, Marc Bailly2, Marianne Bergere3, Robert Wainer2, Vincent Izard4, François Vialard3, Jacqueline Selva3, Florence Boitrelle5. 1. Department of Reproductive Biology, Cytogenetics and Gynaecology, Poissy General Hospital, Poissy, France; Department of Reproductive Biology, Institut Mutualiste Montsouris, Paris, France; EA7404-GIG, UFR des Sciences de la Santé Simone Veil, Université Paris-Saclay, Montigny le Bretonneux, France. 2. Department of Reproductive Biology, Cytogenetics and Gynaecology, Poissy General Hospital, Poissy, France. 3. Department of Reproductive Biology, Cytogenetics and Gynaecology, Poissy General Hospital, Poissy, France; EA7404-GIG, UFR des Sciences de la Santé Simone Veil, Université Paris-Saclay, Montigny le Bretonneux, France. 4. Department of Reproductive Biology, Institut Mutualiste Montsouris, Paris, France. 5. Department of Reproductive Biology, Cytogenetics and Gynaecology, Poissy General Hospital, Poissy, France; EA7404-GIG, UFR des Sciences de la Santé Simone Veil, Université Paris-Saclay, Montigny le Bretonneux, France. Electronic address: florenceboitrelle@yahoo.fr.
Abstract
OBJECTIVE: To assess sperm quality as a function of the sampling site (testis or epididymis) in obstructive azoospermia (OA). MATERIALS AND METHODS: DNA fragmentation rates in spermatozoa sampled from the testis and epididymis (from patients with different etiologies of OA) were assessed in a dUTP nick-end labeling assay. RESULTS: Twenty-one OA patients were included: 5 had congenital bilateral absence of the vas deferens, 8 had genital tract infections, and 8 had idiopathic OA. A total of 8506 spermatozoa sampled from the testis, 18,358 sampled from the caput epididymis, and 18,881 sampled from the corpus/cauda epididymis were assessed. For each patient, spermatozoa from the testis had a lower overall DNA fragmentation rate (6.71% ± 0.75 in average) than epididymal spermatozoa from the caput (14.86% ± 1.89 in average; P = .0007) or the corpus/cauda (32.61% ± 3.11 in average; P < .0001). The DNA fragmentation rates did not differ significantly as a function of the etiology of OA. In this small series, all deliveries were obtained with sperm samples with a low DNA fragmentation rate and delivery rates tended to be higher when testicular sperm (rather than epididymal sperm) was used (35.7% vs 12.1%, respectively; P = .06). CONCLUSION: Our data argue in favor of using testicular sperm (rather than epididymal sperm) for patients with obstructive azoospermia.
OBJECTIVE: To assess sperm quality as a function of the sampling site (testis or epididymis) in obstructive azoospermia (OA). MATERIALS AND METHODS: DNA fragmentation rates in spermatozoa sampled from the testis and epididymis (from patients with different etiologies of OA) were assessed in a dUTP nick-end labeling assay. RESULTS: Twenty-one OA patients were included: 5 had congenital bilateral absence of the vas deferens, 8 had genital tract infections, and 8 had idiopathic OA. A total of 8506 spermatozoa sampled from the testis, 18,358 sampled from the caput epididymis, and 18,881 sampled from the corpus/cauda epididymis were assessed. For each patient, spermatozoa from the testis had a lower overall DNA fragmentation rate (6.71% ± 0.75 in average) than epididymal spermatozoa from the caput (14.86% ± 1.89 in average; P = .0007) or the corpus/cauda (32.61% ± 3.11 in average; P < .0001). The DNA fragmentation rates did not differ significantly as a function of the etiology of OA. In this small series, all deliveries were obtained with sperm samples with a low DNA fragmentation rate and delivery rates tended to be higher when testicular sperm (rather than epididymal sperm) was used (35.7% vs 12.1%, respectively; P = .06). CONCLUSION: Our data argue in favor of using testicular sperm (rather than epididymal sperm) for patients with obstructive azoospermia.
Authors: Sandro C Esteves; Armand Zini; Robert Matthew Coward; Donald P Evenson; Jaime Gosálvez; Sheena E M Lewis; Rakesh Sharma; Peter Humaidan Journal: Andrologia Date: 2020-10-27 Impact factor: 2.775
Authors: Scott J Morin; Brent M Hanson; Caroline R Juneau; Shelby A Neal; Jessica N Landis; Richard T Scott; James M Hotaling Journal: Asian J Androl Date: 2020 Mar-Apr Impact factor: 3.285