Jung-Soo Bae1, Mira Han1, Hee Soon Shin2, Min-Kyoung Kim1, Chang-Yup Shin1, Dong Hun Lee3, Jin Ho Chung4. 1. Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea. 2. Korea Food Research Institute, Seongnam-si, Kyeonggi-do 463-746, Korea. 3. Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea. Electronic address: ivymed27@snu.ac.kr. 4. Department of Dermatology, Seoul National University College of Medicine, 101, Daehak-ro Jongno-gu, Seoul, Korea; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, 101, Daehak-ro Jongno-gu, Seoul, Korea; Instutite of Human-Environment Interface Biology, Seoul National University, 101, Daehak-ro Jongno-gu, Seoul, Korea; SNU Institute on Aging, 101, Daehak-ro Jongno-gu, Seoul, Korea. Electronic address: jhchung@snu.ac.kr.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well. AIM OF THE STUDY: The present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. MATERIALS AND METHODS: Human dermal fibroblasts and Skh-1 hairless mice were irradiated with UV and treated with PLE. Protein and mRNA levels of various target molecules were analyzed by western blotting and quantitative RT-PCR, respectively. Histological changes of mouse skin were analyzed by H&E staining. To elucidate underlying mechanism of PLE, activator protein-1 (AP-1) DNA binding assay and the measurement of reactive oxygen species (ROS) were performed. RESULTS: PLE significantly inhibited basal and UV-induced MMP-1 and MMP-3 expression dose-dependently, and also decreased UV-induced phosphorylation of extracellular signal-regulated kinases and c-Jun N-terminal kinases. This inhibitory effects of PLE on MMP-1 and MMP-3 were mediated by reduction of ROS generation and AP-1 DNA binding activity induced by UV. Furthermore, PLE promoted type I procollagen production irrespective of UV irradiation. In the UV-irradiated animal model, PLE significantly reduced epidermal skin thickness and MMP-13 expression induced by UV. CONCLUSION: Our results demonstrate that PLE has the protective effect against UV-induced dermal matrix damage. Therefore, we suggest that PLE can be a potential agent for prevention of skin aging.
ETHNOPHARMACOLOGICAL RELEVANCE: Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well. AIM OF THE STUDY: The present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin. MATERIALS AND METHODS:Human dermal fibroblasts and Skh-1 hairless mice were irradiated with UV and treated with PLE. Protein and mRNA levels of various target molecules were analyzed by western blotting and quantitative RT-PCR, respectively. Histological changes of mouse skin were analyzed by H&E staining. To elucidate underlying mechanism of PLE, activator protein-1 (AP-1) DNA binding assay and the measurement of reactive oxygen species (ROS) were performed. RESULTS: PLE significantly inhibited basal and UV-induced MMP-1 and MMP-3 expression dose-dependently, and also decreased UV-induced phosphorylation of extracellular signal-regulated kinases and c-Jun N-terminal kinases. This inhibitory effects of PLE on MMP-1 and MMP-3 were mediated by reduction of ROS generation and AP-1 DNA binding activity induced by UV. Furthermore, PLE promoted type I procollagen production irrespective of UV irradiation. In the UV-irradiated animal model, PLE significantly reduced epidermal skin thickness and MMP-13 expression induced by UV. CONCLUSION: Our results demonstrate that PLE has the protective effect against UV-induced dermal matrix damage. Therefore, we suggest that PLE can be a potential agent for prevention of skin aging.
Authors: Young Her; Tae-Kyeong Lee; Jong Dai Kim; Bora Kim; Hyejin Sim; Jae-Chul Lee; Ji Hyeon Ahn; Joon Ha Park; Ji-Won Lee; Junkee Hong; Sung-Su Kim; Moo-Ho Won Journal: Molecules Date: 2020-10-07 Impact factor: 4.411