F Zandona1, H A Soini2, M V Novotny2, E Santiago3, G J Eckert4, J S Preisser5, H K Benecha6, R A Arthur6, D T Zero7. 1. Department of Operative Dentistry, University of North Carolina School of Dentistry at Chapel Hill, Chapel Hill, USA. 2. Department of Chemistry, Institute for Pheromone Research, Indiana University, Bloomington, Indiana, USA. 3. School of Dentistry Research Center, University of Puerto Rico, San Juan, Puerto Rico, USA. 4. Department of Biostatistics, Indiana University School of Medicine, Indianapolis, Indiana, USA. 5. Department of Biostatistics, UNC Gillings School of Global Public Health, Chapel Hill, USA. 6. Department of Preventive and Community Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 7. Department of Preventive and Community Dentistry, Indiana University School of Dentistry, Indianapolis, Indiana, USA.
Abstract
BACKGROUND: This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. METHODS: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. RESULTS: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. CONCLUSIONS: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.
BACKGROUND: This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. METHODS: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. RESULTS: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. CONCLUSIONS: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.
Entities:
Keywords:
Biomarker; Caries-activity; Dental-caries; Gas chromatography-mass spectrometry; Metabolites
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