Minh-Trang Phan1, Sejong Chun2, Sun-Hee Kim2, Alaa Kassim Ali3, Seung-Hwan Lee3, Seokho Kim4, Soo-Hyun Kim5, Duck Cho6. 1. Center for Creative Biomedical Scientists, Chonnam National University, GwangJu, South Korea; Department of Laboratory Medicine, Chonnam National University, GwangJu, South Korea. 2. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, South Korea. 3. Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada. 4. Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea. 5. Department of Laboratory Medicine, Chonnam National University, GwangJu, South Korea. Electronic address: alpinboy@chonnam.ac.kr. 6. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, South Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, South Korea. Electronic address: duck.cho@skku.edu.
Abstract
BACKGROUND: The purpose of this study was to identify CD56bright and CD56dim natural killer (NK) cell subsets and analyze their receptors expression in a healthy Korean population, and to determine whether receptor expression correlates with age, sex, and cytotoxicity. MATERIALS AND METHODS: We performed multicolor flow cytometry assays to analyze the expression of various NK cell receptors (CD16, NKG2A, NKG2C, NKG2D, CD57, DNAM-1, CD8a, CD62L, NKp30, and NKp46) on both CD3-/CD56dim and CD3-/CD56bright NK cells in whole-blood samples from 122 healthy donors. The expression of these receptors was compared according to age (<30years, n=22, 30-60years, n=73 and >60years, n=27) and gender (male, n=61, female, n=61). NK cell cytotoxicity assays were performed with peripheral blood mononuclear cells (PBMCs) from 18 individuals. The results were compared to the expression levels of NKp30 and NKp46 receptors. RESULTS: A normal reference range for NK cell receptor expression in two NK cell subsets was established. NKp46 and NKG2D expression gradually decreased with age (p<0.01 and p<0.05, respectively) whereas NK cell proportion and numbers, frequencies of CD56dim cells, and CD57 expression increased with age (p<0.01 in all cases). Men showed greater NK cell proportion and numbers, frequencies of CD56dim cells, and CD57 expression compared to those of women (p<0.05 and p<0.001; p<0.01 and p<0.01, respectively). Notably, the expression of NKp46 was negatively correlated with NK cell frequency (r=-0.42, p<0.001). Furthermore, NK cell cytotoxicity was found to positively correlate with NCR expression (p=0.02), but not NK cell proportion (p=0.80). CONCLUSION: We have established a profile of NK cell surface receptors for a Korean population, and revealed that age and gender have an effect on the expression of NK cell receptors in the population. Our data might explain why neither NK cell numbers nor proportions correlate with NK cell cytotoxicity.
BACKGROUND: The purpose of this study was to identify CD56bright and CD56dim natural killer (NK) cell subsets and analyze their receptors expression in a healthy Korean population, and to determine whether receptor expression correlates with age, sex, and cytotoxicity. MATERIALS AND METHODS: We performed multicolor flow cytometry assays to analyze the expression of various NK cell receptors (CD16, NKG2A, NKG2C, NKG2D, CD57, DNAM-1, CD8a, CD62L, NKp30, and NKp46) on both CD3-/CD56dim and CD3-/CD56bright NK cells in whole-blood samples from 122 healthy donors. The expression of these receptors was compared according to age (<30years, n=22, 30-60years, n=73 and >60years, n=27) and gender (male, n=61, female, n=61). NK cell cytotoxicity assays were performed with peripheral blood mononuclear cells (PBMCs) from 18 individuals. The results were compared to the expression levels of NKp30 and NKp46 receptors. RESULTS: A normal reference range for NK cell receptor expression in two NK cell subsets was established. NKp46 and NKG2D expression gradually decreased with age (p<0.01 and p<0.05, respectively) whereas NK cell proportion and numbers, frequencies of CD56dim cells, and CD57 expression increased with age (p<0.01 in all cases). Men showed greater NK cell proportion and numbers, frequencies of CD56dim cells, and CD57 expression compared to those of women (p<0.05 and p<0.001; p<0.01 and p<0.01, respectively). Notably, the expression of NKp46 was negatively correlated with NK cell frequency (r=-0.42, p<0.001). Furthermore, NK cell cytotoxicity was found to positively correlate with NCR expression (p=0.02), but not NK cell proportion (p=0.80). CONCLUSION: We have established a profile of NK cell surface receptors for a Korean population, and revealed that age and gender have an effect on the expression of NK cell receptors in the population. Our data might explain why neither NK cell numbers nor proportions correlate with NK cell cytotoxicity.
Authors: Peter J Darlington; Brandon Stopnicki; Tarik Touil; Jean-Sebastien Doucet; Lama Fawaz; Morgan E Roberts; Marie-Noëlle Boivin; Nathalie Arbour; Mark S Freedman; Harold L Atkins; Amit Bar-Or Journal: Front Immunol Date: 2018-05-07 Impact factor: 7.561
Authors: A Oras; B Quirant-Sanchez; D Popadic; S Thunberg; O Winqvist; S Heck; M Cwikowski; D Riemann; B Seliger; E Martinez Caceres; R Uibo; T Giese Journal: Clin Exp Immunol Date: 2020-08-03 Impact factor: 4.330