Cleo L Crunelle1, Michel Yegles2, Mireille De Doncker3, Delphine Cappelle4, Adrian Covaci4, Alexander L N van Nuijs4, Hugo Neels5. 1. Toxicological Center, Department of Pharmaceutical Sciences, University of Antwerp, Wilrijk, Belgium; University Hospital Brussels (UZ Brussel), Department of Psychiatry, Jette, Belgium. Electronic address: cleo.crunelle@gmail.com. 2. Laboratoire National de Santé, Service de Toxicologie, Dudelange, Luxembourg. 3. Toxicology and TDM Laboratory, ZNA Stuivenberg, Antwerp, Belgium. 4. Toxicological Center, Department of Pharmaceutical Sciences, University of Antwerp, Wilrijk, Belgium. 5. Toxicological Center, Department of Pharmaceutical Sciences, University of Antwerp, Wilrijk, Belgium; Toxicology and TDM Laboratory, ZNA Stuivenberg, Antwerp, Belgium.
Abstract
AIMS: Ethyl glucuronide in hair (hEtG) can be used to assess the retrospective consumption of alcohol. A lower cut-off of 7pg/mg hair in the 0-3cm proximal scalp hair segment has been used for repeated alcohol consumption in the previous three months. While a concentration below this cut-off is stated not to contradict self reported abstinence, it is often used to assess whether an individual has remained abstinent in the period prior to hair sampling. Here, we address hEtG concentrations in alcohol consuming individuals and critically evaluate this cut-off value. METHODS: Ten individuals remained abstinent from alcohol for 12 weeks. A lock of hair was cut before the start of the study, and the regrown hairs were cut after twelve weeks of abstinence. Hair EtG concentrations were measured both at baseline and after 12 weeks of abstinence. Study compliance was assessed by urine analysis every 2-3 days by liquid chromatography-tandem mass spectrometry with a lower limit of quantification (LLOQ) of 0.1μg/mL. HEtG concentrations were assessed in the first 3cm hair using gas chromatography-tandem mass spectrometry with an LLOQ of 0.2pg/mg. RESULTS: At the beginning of the study, participants had hEtG concentrations ranging between <LLOQ and 14.0pg/mg hair, matching their pre-study reported alcohol consumption (between 0 to 85g alcohol consumed per week). After 12 weeks of abstinence, only one participant had an hEtG concentration below the LLOQ. Other participants had hEtG concentrations between 0.2 and 4.5pg/mg hair. All urine results were below LLOQ, providing evidence for complete abstinence during the study. DISCUSSION: In participants consuming no alcohol, all but one had low, but measurable hEtG concentrations (up to 4.5pg/mg hair), which was in the participant with the highest pre-study alcohol consumption. As only regrown hairs were cut, it is not likely that this was due to residual EtG from the pre-study period. CONCLUSIONS: Although the number of specimens was low, this study reports measurable hEtG concentrations following total abstinence, although not exceeding the current 7pg/mg cut-off for hair. A suitable sensitive method (GC-MS/MS) is preferred when assessing alcohol abstinence. We propose that the current cut-off of 7pg/mg should be discussed further, and, in view of the small study sample, evaluated using a larger sample size.
AIMS: Ethyl glucuronide in hair (hEtG) can be used to assess the retrospective consumption of alcohol. A lower cut-off of 7pg/mg hair in the 0-3cm proximal scalp hair segment has been used for repeated alcohol consumption in the previous three months. While a concentration below this cut-off is stated not to contradict self reported abstinence, it is often used to assess whether an individual has remained abstinent in the period prior to hair sampling. Here, we address hEtG concentrations in alcohol consuming individuals and critically evaluate this cut-off value. METHODS: Ten individuals remained abstinent from alcohol for 12 weeks. A lock of hair was cut before the start of the study, and the regrown hairs were cut after twelve weeks of abstinence. Hair EtG concentrations were measured both at baseline and after 12 weeks of abstinence. Study compliance was assessed by urine analysis every 2-3 days by liquid chromatography-tandem mass spectrometry with a lower limit of quantification (LLOQ) of 0.1μg/mL. HEtG concentrations were assessed in the first 3cm hair using gas chromatography-tandem mass spectrometry with an LLOQ of 0.2pg/mg. RESULTS: At the beginning of the study, participants had hEtG concentrations ranging between <LLOQ and 14.0pg/mg hair, matching their pre-study reported alcohol consumption (between 0 to 85g alcohol consumed per week). After 12 weeks of abstinence, only one participant had an hEtG concentration below the LLOQ. Other participants had hEtG concentrations between 0.2 and 4.5pg/mg hair. All urine results were below LLOQ, providing evidence for complete abstinence during the study. DISCUSSION: In participants consuming no alcohol, all but one had low, but measurable hEtG concentrations (up to 4.5pg/mg hair), which was in the participant with the highest pre-study alcohol consumption. As only regrown hairs were cut, it is not likely that this was due to residual EtG from the pre-study period. CONCLUSIONS: Although the number of specimens was low, this study reports measurable hEtG concentrations following total abstinence, although not exceeding the current 7pg/mg cut-off for hair. A suitable sensitive method (GC-MS/MS) is preferred when assessing alcohol abstinence. We propose that the current cut-off of 7pg/mg should be discussed further, and, in view of the small study sample, evaluated using a larger sample size.
Authors: Fritz Pragst; Franziska Krumbiegel; Denise Thurmann; Lena Westendorf; Maximilian Methling; André Niebel; Sven Hartwig Journal: Int J Legal Med Date: 2020-01-21 Impact factor: 2.686
Authors: Janique Arnts; Benedict T K Vanlerberghe; Sylvia Roozen; Cleo L Crunelle; Ad A M Masclee; Steven W M Olde-Damink; Ron M A Heeren; Alexander van Nuijs; Hugo Neels; Frederik Nevens; Jef Verbeek Journal: Alcohol Clin Exp Res Date: 2020-12-25 Impact factor: 3.455