Data on early postoperative changes in aqueous monocyte chemoattractant protein-1 levels after phacoemulsification.

The data presented in this article are related to the research article entitled "Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification" (M. Kawai, T. Inoue, M. Inatani, N. Tsuboi, K. Shobayashi, A. Matsukawa, A. Yoshida, H, 2012) [1]. The mean (±SE) aqueous MCP-1 levels (pg/ml) were 31.2±12.5, 1931.2±910.7, 2172.2±1015.7, 3315.4 ±1535.8, 3015.9 ±914.4, 2709.0 ±738.7, 72.8 ±26.9, and 207.1±62.9 at 0, 3, 6, 12, 24, 48, 168, and 720 h after phacoemulsification, respectively. The immunohistochemical analysis showed a number of MCP-1 positive inflammatory cells in the anterior chamber and conjunctiva. There were some MCP-1 positive cells in the corneal endothelium.

Specifications Table

Values of the data

Aqueous level of MCP-1 could be a reliable marker of ocular inflammation after phacoemulsification.

The data are useful to understand the time-dependent changes in ocular inflammation after phacoemulsification.

Methodology can be used for further estimation of other cytokine.

Data

Fig. 1 shows the time course of aqueous MCP-1 levels after phacoemulsification. Levels increased sharply with a peak at 12 h after surgery that gradually decreased for 7 days (168 h). It again increased for 30 days (720 h) as described in our previous report [1]. The mean (±SE) aqueous MCP-1 levels (pg/mL) were 31.2±12.5, 1931.2±910.7, 2172.2±1015.7, 3315.4±1535.8, 3015.9±914.4, 2709.0±738.7, and 72.8±26.9 at 0, 3, 6, 12, 24, 48, 168 h after surgery, respectively. The value at 12 h after surgery was significantly higher (P=0.041) than that of controls. The results of immunohistochemical analyses for MCP-1 are shown in Fig. 2. Six hours after phacoemulsification, many MCP-1-positive cells were found in the anterior chamber and conjunctiva, and MCP-1 immunoreactivities were noted in some corneal endothelial cells. In contrast, no MCP-1-positive cells were observed in control eyes.

Fig. 1

Time-dependent changes in MCP-1 levels after phacoemulsification. Mean±standard error, *P<0.05 using Dunnett׳s test.

Fig. 2

Immunohistochemical analysis for MCP-1 (brown) in anterior eye segments at 6 h after phacoemulsification. Hematoxylin was used as a counter stain. Scale bar, 50 µm.

Experimental design, materials and methods

The animals subjected to surgery were euthanized after collecting aqueous humor 3, 6, 12, 24, 48, 168, and 720 h after surgery (n=4–6), and six control animals were euthanized without surgery. ELISA and immunohistochemical analysis were conducted as described previously [1]. Dunnett׳s test was used for statistical analyses, and P values less than 0.05 were considered significant.

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Subject area	Biology
More specific subject area	MCP-1 after phacoemulsification
Type of data	Figures
How data was acquired	ELISA and Immunohistochemistry
Data format	Analyzed
Experimental factors	Aqueous levels of MCP-1 and localization of MCP-1 positive cells in anterior ocular segment
Experimental features	Time-dependent changes in aqueous MCP-1 level after phacoemulsification
Data source location	Kumamoto University, Kumamoto City, Japan
Data accessibility	The data are within this article