| Literature DB >> 27879222 |
Enrique Sosa1, Rachel Kim1, Ernesto J Rojas1, Linzi Hosohama1, Jon D Hennebold2, Kyle E Orwig3, Amander T Clark4.
Abstract
We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.Entities:
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Year: 2016 PMID： 27879222 PMCID： PMC5123433 DOI： 10.1016/j.scr.2016.09.015
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020