Literature DB >> 27879221

Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.

Anne M Bara1, Angelica Messana1, Amanda Herring1, Dane Z Hazelbaker1, Kevin Eggan2, Lindy E Barrett3.   

Abstract

Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
Copyright © 2016 Helmholtz Zentrum München. Published by Elsevier B.V. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2016        PMID： 27879221     DOI： 10.1016/j.scr.2016.09.008

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  4 in total

1.  Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system.

Authors:  Cuicui Lyu; Jun Shen; Rui Wang; Haihui Gu; Jianping Zhang; Feng Xue; Xiaofan Liu; Wei Liu; Rongfeng Fu; Liyan Zhang; Huiyuan Li; Xiaobing Zhang; Tao Cheng; Renchi Yang; Lei Zhang
Journal:  Stem Cell Res Ther       Date:  2018-04-06       Impact factor: 6.832

2.  Molecular convergence between Down syndrome and fragile X syndrome identified using human pluripotent stem cell models.

Authors:  Sara G Susco; Sulagna Ghosh; Patrizia Mazzucato; Gabriella Angelini; Amanda Beccard; Victor Barrera; Martin H Berryer; Angelica Messana; Daisy Lam; Dane Z Hazelbaker; Lindy E Barrett
Journal:  Cell Rep       Date:  2022-09-06       Impact factor: 9.995

3.  A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease.

Authors:  Dane Z Hazelbaker; Amanda Beccard; Anne M Bara; Nicole Dabkowski; Angelica Messana; Patrizia Mazzucato; Daisy Lam; Danielle Manning; Kevin Eggan; Lindy E Barrett
Journal:  Stem Cell Reports       Date:  2017-10-10       Impact factor: 7.765

4.  NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells.

Authors:  Qisheng Zuo; Chen Zhang; Kai Jin; Jin Jing; Changhua Sun; Mahmoud F Ahmed; Jiuzhou Song; Yani Zhang; Guohong Chen; Bichun Li
Journal:  Cell Biosci       Date:  2018-06-19       Impact factor: 7.133
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.