Literature DB >> 27879215

A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition.

Ho Sun Jung1, Gene Uenishi2, Akhilesh Kumar1, Mi Ae Park1, Matt Raymond1, Dustin Fink1, Ethan McLeod1, Igor Slukvin3.   

Abstract

Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VEC+ endothelial cells express tdTomato and CD43+ hematopoietic progenitors express eGFP.
Copyright © 2016 Helmholtz Zentrum München. Published by Elsevier B.V. All rights reserved.

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Year:  2016        PMID： 27879215      PMCID： PMC5123598          DOI： 10.1016/j.scr.2016.09.004

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  14 in total

1.  Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.

Authors:  Kyung-Dal Choi; Maxim Vodyanik; Igor I Slukvin
Journal:  Nat Protoc       Date:  2011-02-17       Impact factor: 13.491

2.  Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells.

Authors:  Amita Tiyaboonchai; Helen Mac; Razveen Shamsedeen; Jason A Mills; Siddarth Kishore; Deborah L French; Paul Gadue
Journal:  Stem Cell Res       Date:  2014-02-21       Impact factor: 2.020

3.  Targeted null-mutation in the vascular endothelial-cadherin gene impairs the organization of vascular-like structures in embryoid bodies.

Authors:  D Vittet; T Buchou; A Schweitzer; E Dejana; P Huber
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

4.  Embryonic stem cell lines derived from human blastocysts.

Authors:  J A Thomson; J Itskovitz-Eldor; S S Shapiro; M A Waknitz; J J Swiergiel; V S Marshall; J M Jones
Journal:  Science       Date:  1998-11-06       Impact factor: 47.728

5.  Expression on blood cells of sialophorin, the surface glycoprotein that is defective in Wiskott-Aldrich syndrome.

Authors:  E Remold-O'Donnell; C Zimmerman; D Kenney; F S Rosen
Journal:  Blood       Date:  1987-07       Impact factor: 22.113

6.  Expression of CD43 on murine and human pluripotent hematopoietic stem cells.

Authors:  T Moore; S Huang; L W Terstappen; M Bennett; V Kumar
Journal:  J Immunol       Date:  1994-12-01       Impact factor: 5.422

7.  Tenascin C promotes hematoendothelial development and T lymphoid commitment from human pluripotent stem cells in chemically defined conditions.

Authors:  Gene Uenishi; Derek Theisen; Jeong-Hee Lee; Akhilesh Kumar; Matt Raymond; Maxim Vodyanik; Scott Swanson; Ron Stewart; James Thomson; Igor Slukvin
Journal:  Stem Cell Reports       Date:  2014-10-23       Impact factor: 7.765

8.  A novel endothelial-specific membrane protein is a marker of cell-cell contacts.

Authors:  M G Lampugnani; M Resnati; M Raiteri; R Pigott; A Pisacane; G Houen; L P Ruco; E Dejana
Journal:  J Cell Biol       Date:  1992-09       Impact factor: 10.539

9.  Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators.

Authors:  Irina Elcheva; Vera Brok-Volchanskaya; Akhilesh Kumar; Patricia Liu; Jeong-Hee Lee; Lilian Tong; Maxim Vodyanik; Scott Swanson; Ron Stewart; Michael Kyba; Eduard Yakubov; John Cooke; James A Thomson; Igor Slukvin
Journal:  Nat Commun       Date:  2014-07-14       Impact factor: 14.919

10.  Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor.

Authors:  Stanislav Rybtsov; Antoniana Batsivari; Kateryna Bilotkach; Daria Paruzina; Jordi Senserrich; Oleg Nerushev; Alexander Medvinsky
Journal:  Stem Cell Reports       Date:  2014-08-28       Impact factor: 7.765
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  4 in total

1.  Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions.

Authors:  Gene I Uenishi; Ho Sun Jung; Igor I Slukvin
Journal:  Methods Mol Biol       Date:  2022

2.  Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells.

Authors:  Akhilesh Kumar; Saritha S D'Souza; Gene Uenishi; Mi Ae Park; Jeong Hee Lee; Igor I Slukvin
Journal:  Bio Protoc       Date:  2020-07-05

3.  NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells.

Authors:  Gene I Uenishi; Ho Sun Jung; Akhilesh Kumar; Mi Ae Park; Brandon K Hadland; Ethan McLeod; Matthew Raymond; Oleg Moskvin; Catherine E Zimmerman; Derek J Theisen; Scott Swanson; Owen J Tamplin; Leonard I Zon; James A Thomson; Irwin D Bernstein; Igor I Slukvin
Journal:  Nat Commun       Date:  2018-05-08       Impact factor: 14.919

Review 4.  Understanding the Journey of Human Hematopoietic Stem Cell Development.

Authors:  Akhilesh Kumar; Saritha S D'Souza; Abir S Thakur
Journal:  Stem Cells Int       Date:  2019-05-06       Impact factor: 5.443
  4 in total

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