Literature DB >> 27876938

Polymerase chain reaction (PCR) based amplification of hmcp3 and hmcp6 cysteine protease genes of Haemonchus contortus from small ruminants.

A Latchumikanthan1, A Prasad1, M Sankar2, Pesingi Pavan Kumar3, M V Jithin4, M Aravind1.   

Abstract

Haemonchus contortus is a haematophagous nematode in small ruminants population and causes anaemia, weakness and mortality especially in young animals. In the present study, hmcp3 partial gene of 836 bp and hmcp6 full length gene of 1041 bp were amplified from the cDNA of Bareilly isolate of adult male H. contortus by polymerase chain reaction. Further gene characterization and expression studies are warranted to know the immunoprophylactic potential of hmcp3 and hmcp6 proteins of H. contortus.

Entities:  

Keywords:  Cysteine protease; Haemonchus contortus; PCR; hmcp3; hmcp6

Year:  2015        PMID： 27876938      PMCID： PMC5118305          DOI： 10.1007/s12639-015-0679-8

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN： 0971-7196


  13 in total

Review 1.  Proteinases and associated genes of parasitic helminths.

Authors:  J Tort; P J Brindley; D Knox; K H Wolfe; J P Dalton
Journal:  Adv Parasitol       Date:  1999       Impact factor: 3.870

Review 2.  Drug resistance in veterinary helminths.

Authors:  Adrian J Wolstenholme; Ian Fairweather; Roger Prichard; Georg von Samson-Himmelstjerna; Nicholas C Sangster
Journal:  Trends Parasitol       Date:  2004-10

3.  Pathophysiology of single and challenge infections of Haemonchus contortus in Merino sheep: studies on red cell kinetics and the "self-cure" phenomenon.

Authors:  J D Dargie; E W Allonby
Journal:  Int J Parasitol       Date:  1975-04       Impact factor: 3.981

4.  Protection induced by vaccinating sheep with a thiol-binding extract of Haemonchus contortus membranes is associated with its protease components.

Authors:  D P Knox; S K Smith; D L Redmond; W D Smith
Journal:  Parasite Immunol       Date:  2005-04       Impact factor: 2.280

5.  A tissue specific approach for analysis of membrane and secreted protein antigens from Haemonchus contortus gut and its application to diverse nematode species.

Authors:  A Rehman; D P Jasmer
Journal:  Mol Biochem Parasitol       Date:  1998-11-30       Impact factor: 1.759

6.  Molecular cloning and characterization of gut-derived cysteine proteinases associated with a host protective extract from Haemonchus contortus.

Authors:  P J Skuce; D L Redmond; S Liddell; E M Stewart; G F Newlands; W D Smith; D P Knox
Journal:  Parasitology       Date:  1999-10       Impact factor: 3.234

7.  Characterization of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode.

Authors:  D P Knox; D L Redmond; D G Jones
Journal:  Parasitology       Date:  1993-05       Impact factor: 3.234

8.  Cloning and characterization of a microsomal aminopeptidase from the intestine of the nematode Haemonchus contortus.

Authors:  T S Smith; M Graham; E A Munn; S E Newton; D P Knox; W J Coadwell; D McMichael-Phillips; H Smith; W D Smith; J J Oliver
Journal:  Biochim Biophys Acta       Date:  1997-04-04

9.  Haemonchus contortus: identification of proteases with diverse characteristics in adult worm excretory-secretory products.

Authors:  F N Karanu; F R Rurangirwa; T C McGuire; D P Jasmer
Journal:  Exp Parasitol       Date:  1993-11       Impact factor: 2.011

10.  Protection studies with integral membrane fractions of Haemonchus contortus.

Authors:  W D Smith; S K Smith; J M Murray
Journal:  Parasite Immunol       Date:  1994-05       Impact factor: 2.280
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