Juan Bugueño1, Weihua Li1, Pinky Salat1, Ling Qin2, Sunday O Akintoye3. 1. Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA. 2. Department of Orthopedics School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. 3. Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: Akintoye@upenn.edu.
Abstract
OBJECTIVES: Mesenchymal stem cells (MSCs) offer a promising therapy in dentistry because of their multipotent properties. Selecting donor MSCs is crucial because Beagle dogs (canines) commonly used in preclinical studies have shown variable outcomes, and it is unclear whether canine MSCs (cMSCs) are skeletal site specific. This study tested whether jaw and long bone cMSCs have disparate in vitro and in vivo multilineage differentiation capabilities. STUDY DESIGN: Primary cMSCs were isolated from the mandible (M-cMSCs) and femur (F-cMSCs) of four healthy Beagle dogs. The femur served as the non-oral control. Clonogenic and proliferative abilities were assessed. In vitro osteogenic, chondrogenic, adipogenic, and neural multilineage differentiation were correlated with in vivo bone regeneration and potential for clinical applications. RESULTS: M-cMSCs displayed two-fold increase in clonogenic and proliferative capacities relative to F-cMSCs (P = .006). M-cMSCs in vitro osteogenesis based on alkaline phosphatase (P = .04), bone sialoprotein (P = .05), and osteocalcin (P = .03), as well as adipogenesis (P = .007) and chondrogenesis (P = .009), were relatively higher and correlated with enhanced M-cMSC bone regenerative capacity. Neural expression markers, nestin and βIII-tubulin, were not significantly different. CONCLUSIONS: The enhanced differentiation and bone regenerative capacity of mandible MSCs may make them favorable donor graft materials for site-specific jaw bone regeneration.
OBJECTIVES: Mesenchymal stem cells (MSCs) offer a promising therapy in dentistry because of their multipotent properties. Selecting donor MSCs is crucial because Beagle dogs (canines) commonly used in preclinical studies have shown variable outcomes, and it is unclear whether canine MSCs (cMSCs) are skeletal site specific. This study tested whether jaw and long bone cMSCs have disparate in vitro and in vivo multilineage differentiation capabilities. STUDY DESIGN: Primary cMSCs were isolated from the mandible (M-cMSCs) and femur (F-cMSCs) of four healthy Beagle dogs. The femur served as the non-oral control. Clonogenic and proliferative abilities were assessed. In vitro osteogenic, chondrogenic, adipogenic, and neural multilineage differentiation were correlated with in vivo bone regeneration and potential for clinical applications. RESULTS: M-cMSCs displayed two-fold increase in clonogenic and proliferative capacities relative to F-cMSCs (P = .006). M-cMSCs in vitro osteogenesis based on alkaline phosphatase (P = .04), bone sialoprotein (P = .05), and osteocalcin (P = .03), as well as adipogenesis (P = .007) and chondrogenesis (P = .009), were relatively higher and correlated with enhanced M-cMSC bone regenerative capacity. Neural expression markers, nestin and βIII-tubulin, were not significantly different. CONCLUSIONS: The enhanced differentiation and bone regenerative capacity of mandible MSCs may make them favorable donor graft materials for site-specific jaw bone regeneration.
Authors: Christine M Reich; Oksana Raabe; Sabine Wenisch; Philip S Bridger; Martin Kramer; Stefan Arnhold Journal: Vet Res Commun Date: 2012-03-04 Impact factor: 2.459
Authors: Ana Ivanovska; Mengyu Wang; Tarlan Eslami Arshaghi; Georgina Shaw; Joel Alves; Andrew Byrne; Steven Butterworth; Russell Chandler; Laura Cuddy; James Dunne; Shane Guerin; Rob Harry; Aidan McAlindan; Ronan A Mullins; Frank Barry Journal: Front Vet Sci Date: 2022-06-10