Noemí Arias1, Catalina Picó2, M Teresa Macarulla1, Paula Oliver2, Jonatan Miranda1, Andreu Palou2, María P Portillo1. 1. Department of Nutrition and Food Science, Nutrition and Obesity Group, University of the Basque Country (UPV/EHU), Lucio Lascaray Center and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Vitoria, Spain. 2. Laboratory of Molecular Biology, Nutrition and Biotechnology, University of the Balearic Islands and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain.
Abstract
OBJECTIVE: To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect. METHODS: Thirty-six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group). RESULTS: After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox-2 mRNA level in the RSV + Q group, and UCP1 protein expression in the RSV and the RSV + Q groups. CONCLUSIONS: This study demonstrated that the RSV + Q combination produces a brown-like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.
OBJECTIVE: To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect. METHODS: Thirty-six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group). RESULTS: After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox-2 mRNA level in the RSV + Q group, and UCP1 protein expression in the RSV and the RSV + Q groups. CONCLUSIONS: This study demonstrated that the RSV + Q combination produces a brown-like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.
Authors: F Concha; G Prado; J Quezada; A Ramirez; N Bravo; C Flores; J J Herrera; N Lopez; D Uribe; L Duarte-Silva; P Lopez-Legarrea; Diego F Garcia-Diaz Journal: Rev Endocr Metab Disord Date: 2019-06 Impact factor: 6.514
Authors: Emily L Rossi; Subreen A Khatib; Steven S Doerstling; Laura W Bowers; Melissa Pruski; Nikki A Ford; Randolph D Glickman; Mengmeng Niu; Peiying Yang; Zhengrong Cui; John DiGiovanni; Stephen D Hursting Journal: Mol Carcinog Date: 2017-12-01 Impact factor: 4.784