Literature DB >> 2787214

Stability of recombinant human epidermal growth factor in various solutions.

F Araki, H Nakamura, N Nojima, K Tsukumo, S Sakamoto.   

Abstract

The stability of recombinant human epidermal growth factor (hEGF) in various solutions was examined. hEGF degraded spontaneously and temperature-dependently to several degradation products in phosphate buffered saline or in 0.1 N acetic acid. The enzymatic degradation was observed in human serum or in pepsin/HCl solution. The structure and biological activities of these compounds were examined. The results suggest that the Asp11 and Trp50 residues are important for the receptor binding.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2787214     DOI： 10.1248/cpb.37.404

Source DB:  PubMed          Journal:  Chem Pharm Bull (Tokyo)        ISSN： 0009-2363            Impact factor:   1.645


  7 in total

1.  Chemical pathways of peptide degradation. II. Kinetics of deamidation of an asparaginyl residue in a model hexapeptide.

Authors:  K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1990-07       Impact factor: 4.200

2.  Spontaneous degradation of polypeptides at aspartyl and asparaginyl residues: effects of the solvent dielectric.

Authors:  T V Brennan; S Clarke
Journal:  Protein Sci       Date:  1993-03       Impact factor: 6.725

Review 3.  Protein damage and methylation-mediated repair in the erythrocyte.

Authors:  P Galletti; D Ingrosso; C Manna; G Clemente; V Zappia
Journal:  Biochem J       Date:  1995-03-01       Impact factor: 3.857

4.  Potency and stability of C terminal truncated human epidermal growth factor.

Authors:  D P Calnan; A Fagbemi; J Berlanga-Acosta; T Marchbank; T Sizer; K Lakhoo; A D Edwards; R J Playford
Journal:  Gut       Date:  2000-11       Impact factor: 23.059

5.  Urinary excretion of epidermal growth factor and rapid loss of kidney function.

Authors:  Jon Viljar Norvik; Laura R Harskamp; Viji Nair; Kerby Shedden; Marit D Solbu; Bjørn O Eriksen; Matthias Kretzler; Ron T Gansevoort; Wenjun Ju; Toralf Melsom
Journal:  Nephrol Dial Transplant       Date:  2021-09-27       Impact factor: 5.992

6.  Chemical pathways of peptide degradation. VII. Solid state chemical instability of an aspartyl residue in a model hexapeptide.

Authors:  C Oliyai; J P Patel; L Carr; R T Borchardt
Journal:  Pharm Res       Date:  1994-06       Impact factor: 4.200

7.  Crystal structure and mechanism of human carboxypeptidase O: Insights into its specific activity for acidic residues.

Authors:  Maria C Garcia-Guerrero; Javier Garcia-Pardo; Esther Berenguer; Roberto Fernandez-Alvarez; Gifty B Barfi; Peter J Lyons; Francesc X Aviles; Robert Huber; Julia Lorenzo; David Reverter
Journal:  Proc Natl Acad Sci U S A       Date:  2018-04-10       Impact factor: 11.205
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.