Literature DB >> 27871465

Identification of genes for normalization of real-time RT-PCR data in placental tissues from intrahepatic cholestasis of pregnancy.

Yanli Li1, Huifang Lu1, Yizhen Ji1, Sufang Wu2, Yongbin Yang3.   

Abstract

The selection of suitable reference genes is crucial for proper interpretation of RT-qPCR data. To date, a systematic screening for reference genes in placental tissues from intrahepatic cholestasis of pregnancy (ICP) is missing. Eighteen candidate reference genes were investigated to determine their applicability by descriptive statistics and published algorithms. HPRT1, YWHAZ and PUM1 were found to be the most stable genes and could be used for normalization in gene expression study of placentas from ICP. The combination of HPRT1 and YWHAZ is sufficient and the use of all three genes should be recommended for an improved reliability of normalization.
Copyright © 2016 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Intrahepatic cholestasis of pregnancy; Placenta; Quantitative real-time reverse transcription polymerase chain reaction; Reference gene

Mesh：

Substances：

Year:  2016        PMID： 27871465     DOI： 10.1016/j.placenta.2016.10.017

Source DB:  PubMed          Journal:  Placenta        ISSN： 0143-4004            Impact factor:   3.481


  6 in total

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