Hueiwang Anna Jeng1, Chih-Hong Pan2, Mu-Rong Chao3, Chien-Chih Chiu4, Guodong Zhou5, Chon-Kit Chou6, Wen-Yi Lin7. 1. Old Dominion University, Norfolk, USA (College of Health Sciences, School of Community and Environmental Health). hjeng@odu.edu. 2. Council of Labor Affairs, Executive Yuan, Taipei, Taiwan (Institute of Occupational Safety and Health). chpan@mail.ilosh.gov.tw. 3. Chung Shan Medical University, Taichung, Taiwan (Department of Occupational Safety and Health). mrchao@csmu.edu.tw. 4. Kaohsiung Medical University, Kaohsiung, Taiwan (College of Health Sciences, Department of Medical Laboratory Science and Biotechnology). cchiu@kmu.edu.tw. 5. Texas A&M Health Science Center, Houston, USA (Institute of Biosciences and Technology, Center for Cancer and Stem Cell Biology). gzhou@ibt.tamhsc.edu. 6. Kaohsiung Medical University, Kaohsiung, Taiwan (College of Health Sciences, Department of Medical Laboratory Science and Biotechnology). woodnettle2002@gmail.com. 7. Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan (Department of Occupational Medicine and Internal Medicine). 810064@ms.kmuh.org.tw.
Abstract
OBJECTIVES: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. MATERIAL AND METHODS: The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. RESULTS: The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. CONCLUSIONS: Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915-926. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.
OBJECTIVES: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. MATERIAL AND METHODS: The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. RESULTS: The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. CONCLUSIONS: Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915-926. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.
Entities:
Keywords:
DNA damage; DNA fragmentation; DNA integrity; bulky DNA adducts; semen quality; sperm
Authors: Sandro C Esteves; Armand Zini; Robert Matthew Coward; Donald P Evenson; Jaime Gosálvez; Sheena E M Lewis; Rakesh Sharma; Peter Humaidan Journal: Andrologia Date: 2020-10-27 Impact factor: 2.775